Data/hora: 09/06/2024 04:39:40

Provedor de dados: 	27	
País: 	Belgium	
Título: 	The nuclear rDNA region of &lt;i&gt;Gyrodactylus arcuatus&lt;/i&gt; and &lt;i&gt;G. branchicus&lt;/i&gt; (Monogenea: Gyrodactylidae)	
Autores: 	Zietara, M.S.; Arndt, A.; Geets, A.; Hellemans, B.; Volckaert, F.A.M.J.	
Data: 	2000	
Ano: 	2000	
Palavras-chave: 	DNA; Molecular structure; Nucleotide sequence; Taxonomy; Gasterosteus aculeatus.	
Resumo: 	The primary structure of the ribosomal DNA internal transcribed spacers (ITS-1 and ITS-2) and 5.8S rRNA gene were used to characterize and identify 2 monogenean species of &lt;i&gt;Gyrodactylus&lt;/i&gt; living externally on the threespine stickleback (&lt;i&gt;Gasterosteus aculeatus&lt;/i&gt;). The ITS region was amplified by PCR from freshwater, brackish, and marine isolates of &lt;i&gt;Gyrodactylus arcuatus&lt;/i&gt; and &lt;i&gt;G. branchicus&lt;/i&gt;, and the ends of the coding regions were identified by comparative alignment. No intraspecific and very low interspecific variation were observed in the 5.8S rRNA gene; high inter- and low intraspecific variation were revealed in the ITS-1 and ITS-2 regions. The morphological species identification was in all cases confirmed by the molecular identification. Intraspecifically, samples from 2 locations in the North Sea could be differentiated, but the Baltic sample resembled North Sea genotypes. Our approach offers perspectives for a multimetric genetical, morphometrical, and ecological taxonomy of the genus &lt;i&gt;Gyrodactylus&lt;/i&gt;.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.vliz.be/nl/open-marien-archief?module=ref&refid=8482	
Relação: 	info:eu-repo/semantics/altIdentifier/wos/000166184200035	
Fonte: 	%3Ci%3EJ.+Parasitol.+86%286%29%3C%2Fi%3E%3A+1368-1373.+%3Ca+href%3D%22https%3A%2F%2Fhdl.handle.net%2F10.2307%2F3285031%22+target%3D%22_blank%22%3Ehttps%3A%2F%2Fhdl.handle.net%2F10.2307%2F3285031%3C%2Fa%3E	
Fonte: 	info:eu-repo/semantics/restrictedAccess