Data/hora: 01/06/2024 11:24:16

Provedor de dados: 	3	
País: 	Brazil	
Título: 	Evaluation of a simplified key for the identification of coagulase-positive Staphylococcus isolated from bovine mastitis - doi: 10.4025/actascibiolsci.v32i4.6276

Evaluation of a simplified key for the identification of coagulase-positive Staphylococcus isolated from bovine mastitis - doi: 10.4025/actascibiolsci.v32i4.6276	
Autores: 	Costa, Geraldo Márcio da; UFLA; Paiva, Luciano Vilela; UFLA; Piccoli, Roberta Hilsdorf; Universidade Federal de Lavras; Figueiredo, Demétrio Junqueira; Universidade Federal de Lavras; Pereira, Ulisses de Pádua; UFLA; Silva, Nivaldo da; EV-UFMG.	
Data: 	2010-11-03	
Ano: 	2010	
Palavras-chave: 	5.05.02.03-4 mastitis pathogens; Characterization; Coagulase test; PCR; Biochemical tests Doenças Infecciosas dos Animais mastitis pathogens; Characterization; Coagulase test; PCR; Biochemical tests.	
Resumo: 	Evaluation of a simplified key for the identification of coagulase-positive Staphylococcus isolated from bovine mastitis. Three hundred fourty four strains of coagulase-positive Staphylococcus (CPS), isolated from mastitis cases, underwent phenotypic and genotypic tests to evaluate the efficiency of a simplified key, based on phenotypic tests for the discrimination of these microorganisms. The tests consisted of amplification of the femA gene and hemolysis in blood agar, production of acetoin and fermentation of maltose, mannitol and trehalose. Strains that showed negative results in the amplification test of the femA gene or that were not identified as Staphylococcus aureus (S. aureus) by phenotypic tests were tested with the APISTAPH kit (Biomériux-France), for precise identification of species. Phenotypic tests revealed 338 strains (98.25%) as S. aureus, three strains (0.86%) as Staphylococcus hyicus, and three microorganisms (0.86%) as Staphylococcus intermedius. PCR demonstrated that 338 (98.25%) strains belonged to the S. aureus species, confirming the results for 336 strains from 338 identified, through a simplified phenotypic key. A high rate of correlation (98.83%) was verifeid between the results of genotypic and phenotypic tests for the identification of S. aureus, demonstrating the applicability of the proposed key, for the discrimination of this microorganism in CPS isolated from bovine mastitis.	
Tipo: 	Pesquisa laboratorial	
Idioma: 	Português	
Identificador: 	http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/6276

10.4025/actascibiolsci.v32i4.6276	
Editor: 	Universidade Estadual De Maringá	
Formato: 	application/pdf

application/pdf	
Fonte: 	Acta Scientiarum. Biological Sciences; v. 32, n. 4 (2010); 403-406

Acta Scientiarum. Biological Sciences; v. 32, n. 4 (2010); 403-406	
Fonte: 	DECLARATION OF ORIGINALITY AND CESSATION OF COPYRIGHTI Declare that current article is original and has not been submitted for publication, in part or in whole, to any other national or international journal. I also declare that once published in the Acta Scientiarum. Biological Sciences, a publication of the Universidade Estadual de Maringá, it will not be submitted by me or by any co-author to any other scientific communication mean. In my name and in the name of co-authors, I shall cede the copyright of the above mentioned article to the Universidade Estadual de Maringá and I declare that I know that the non-observance of this norm may make me liable for the penalties contemplated in the Law for the Protection of Authors’ Rights (Act 9609 of the 19th February 1998).

DECLARAÇÃO DE ORIGINALIDADE E CESSÃO DE DIREITOS AUTORAIS  Declaro que o presente artigo é original, não tendo sido submetido à publicação em qualquer outro periódico nacional ou internacional, quer seja em parte ou em sua totalidade. Declaro, ainda, que uma vez publicado na revista Acta Scientiarum. Biological Sciences, editada pela Universidade Estadual de Maringá, o mesmo jamais será submetido por mim ou por qualquer um dos demais co-autores a qualquer outro meio de divulgação científica. Através deste instrumento, em meu nome e em nome dos demais co-autores, porventura existentes, cedo os direitos autorais do referido artigo à Universidade Estadual de Maringá e declaro estar ciente de que a não observância deste compromisso submeterá o infrator a sanções e penas previstas na Lei de Proteção de Direitos Autorias (Nº9609, de 19/02/98).