Data/hora: 11/06/2024 12:13:48

Provedor de dados: | 3
País: | Brazil
Título: | Screening of whole yeast free-cells and optimization of pH and temperature for fructooligosaccharides production
Autores: | Deffert, Flávia; Agustini, Bruna Carla; Picheth, Geraldo; Bonfim, Tania Maria Bordin.
Data: | 2017-06-16
Ano: | 2017
Palavras-chave: | 2.12.00.00-9 prebiotics; Biotransformation; Identification; PCR. Microbiologia.
Resumo: | Fructooligosaccharides are catalyzed by β–fructofuranosidase enzyme, produced by many microorganisms. However, in order to achieve a more profitable, low time-consuming process with lower cost, researchers have sought alternatives. This study aimed to select and identify yeasts able to produce fructooligosaccharides and evaluate the influence of pH and temperature on their synthesis. Yeast suspensions, solutions of 500 g L-1 sucrose and three values of pH (4.5, 5.5, and 6.5) and temperature (40, 50, and 60ºC) were tested. Yeast species were identified by molecular techniques. Among 141 yeast isolates from grapes, 65 were able to synthesize fructooligosaccharides. The maximum concentration of fructooligosaccharides was 4.8% (w v-1), and Saccharomyces cerevisiae 222 produced 1-kestose and nystose.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês

Identificador:

http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/34140

10.4025/actascibiolsci.v39i2.34140

Editor: | Universidade Estadual De Maringá

Relação:

http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/34140/pdf

http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/downloadSuppFile/34140/13728

Formato: | application/pdf

Fonte:

Acta Scientiarum. Biological Sciences; v. 39, n. 2 (2017); 189-194

Acta Scientiarum. Biological Sciences; v. 39, n. 2 (2017); 189-194

1807-863X

1679-9283

Fonte: | Direitos autorais 2017 Acta Scientiarum. Biological Sciences