Data/hora: 11/06/2024 01:27:09

Provedor de dados: 	3	
País: 	Brazil	
Título: 	Extraction, physical-chemical characterization and in vitro inhibitory potential of thrombin generation of crude sulfated polysaccharides from Brazilian tropical seaweeds	
Autores: 	Rodrigues, José Ariévilo Gurgel; Barcellos, Priscila Gomes; Glauser, Bianca Fernandes; Quinderé, Ana Luíza Gomes; Benevides, Norma Maria Barros; Tovar, Ana Maria Freire; Mourão, Paulo Antônio de Souza.	
Data: 	2018-03-28	
Ano: 	2018	
Palavras-chave: 	Marine algae; Polyglycans; Coagulation tests; Thrombin.	
Resumo: 	The biotechnological value of macroalgae for screening assays of thrombin generation-TG using sulfated polysaccharides-SPs as substitutes to heparin has been poorly explored. Five Brazilian species of macroalgae (Gracilaria birdiae, Acanthophora muscoides, Halymenia sp., Caulerpa cupressoides and C. racemosa) were analyzed and compared for their abundance, physical-chemical characteristics and in vitro anticoagulant assays of activated partial thromboplastin time-APTT, prothrombin time-PT and TG. Papain extraction yielded (p &lt; 0.001) from 0.66±0.03% (C. racemosa) to 41.60±1.10% (Halymenia sp.) of crude SPs  varying sulfate (8.41-42.60%) and total sugars (47.80-70.53%). Crude SPs showed difference in mobility and resolution pattern by agarose electrophoresis, while polyacrylamide analysis revealed SPs of &gt; 100 kDa. These procedures, combined with the use of Stains-All, also indicated nonSPs. APTTs ranged from 2.81 (A. muscoides) to 21.30 IU (Halymenia sp.) vs. heparin (193 IU), and were dependent on sulfation of the crude SPs. PT was not altered. With respect to TG assay, crude SPs modified concentration-dependent and independently from molecular mass TG by both intrinsic/extrinsic pathways in 60-fold diluted human plasma, with total intrinsic inactivation using crude SPs from A. muscoides in parallel to heparin (p &lt; 0.05). Thrombosis in vitro is differentially modulated by distinct crude SPs from Brazilian seaweeds.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/34463

10.4025/actascibiolsci.v40i1.34463	
Editor: 	Universidade Estadual De Maringá	
Relação: 	http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/34463/pdf

http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/downloadSuppFile/34463/13829	
Formato: 	application/pdf	
Fonte: 	Acta Scientiarum. Biological Sciences; v. 40 (2018): Publicação Contínua; e34463

Acta Scientiarum. Biological Sciences; v. 40 (2018): Publicação Contínua; e34463

1807-863X

1679-9283	
Fonte: 	Direitos autorais 2018 Acta Scientiarum. Biological Sciences