Data/hora: 09/06/2024 11:55:01

Provedor de dados: | 3
País: | Brazil
Título: | Comparative study of sulfated polysaccharides from Caulerpa spp. (Chlorophyceae). Biotechnological tool for species identification? - doi: 10.4025/actascibiolsci.v34i4.8976
Autores: | Rodrigues, José Ariévilo Gurgel; Rede Nordeste de Biotecnologia, Universidade Estadual do Ceará; Quinderé, Ana Luíza Gomes; Universidade Federal do Ceará; Queiroz, Ismael Nilo Lino de; Universidade Federal do Ceará; Coura, Chistiane Oliveira; Universidade Federal do Ceará; Benevides, Norma Maria Barros; Universidade Federal do Ceará.
Data: | 2012-09-04
Ano: | 2012
Palavras-chave: | 2.08.01.00-9 chlorophyta; Sulfated polysaccharides; Molecular markers; APTT test 2.08.01.00-9.
Resumo: | Studies on macromolecules isolated from marine algae suggested sulfated polysaccharides (SPs) as possible molecular markers for species. We evaluated isolated and fractionated SPs from the green marine algae Caulerpa cupressoides, C. prolifera and C. racemosa collected at Pacheco  Beach, as possible taxonomic molecular indicators. Total SPs were extracted with papain in 100 mM sodium acetate buffer (pH 5.0) containing cysteine and EDTA (both 5 mM), followed by ion-exchange chromatography on DEAE-cellulose using a NaCl gradient. The obtained fractions were analyzed by 0.5% agarose gel electrophoresis. Anticoagulant assays employing normal human plasma and standard heparin (193 IU mg-1) by the activated partial thromboplastin time (APTT) test were also performed as comparison parameters. Low yields, and similar chromatographic profiles were found among species’ SPs, but electrophoresis revealed distinct SPs resolution patterns. The changes in APTT of SP fractions were dependent on charge density as showed by electrophoresis profiles. Activities were 17.37 (C. cupressoides), 22.17 (C. racemosa) and 25.64 (C. prolifera) IU mg-1, respectively, similar to a previous study using the first and second species. The results suggest that comparative studies of SPs isolated from seaweeds may be an important tool for the identification of Caulerpaceae.
Tipo: | Bioquímica comparativa de algas
Idioma: | Inglês

Identificador:

http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/8976

10.4025/actascibiolsci.v34i4.8976

Editor: | Universidade Estadual De Maringá

Formato:

application/pdf

application/pdf

Fonte:

Acta Scientiarum. Biological Sciences; v. 34, n. 4 (2012); 381-389

Acta Scientiarum. Biological Sciences; v. 34, n. 4 (2012); 381-389

Fonte:

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DECLARAÇÃO DE ORIGINALIDADE E CESSÃO DE DIREITOS AUTORAIS  Declaro que o presente artigo é original, não tendo sido submetido à publicação em qualquer outro periódico nacional ou internacional, quer seja em parte ou em sua totalidade. Declaro, ainda, que uma vez publicado na revista Acta Scientiarum. Biological Sciences, editada pela Universidade Estadual de Maringá, o mesmo jamais será submetido por mim ou por qualquer um dos demais co-autores a qualquer outro meio de divulgação científica. Através deste instrumento, em meu nome e em nome dos demais co-autores, porventura existentes, cedo os direitos autorais do referido artigo à Universidade Estadual de Maringá e declaro estar ciente de que a não observância deste compromisso submeterá o infrator a sanções e penas previstas na Lei de Proteção de Direitos Autorias (Nº9609, de 19/02/98).