Data/hora: 06/06/2024 08:31:35

Provedor de dados: | 3
País: | Brazil
Título: | Micropropagation of Brasilidium forbesii (Orchidaceae) through transverse and longitudinal thin cell layer culture
Autores: | Gomes, Lucas Roberto Pereira; Franceschi, Cristina do Rosário Batista; Ribas, Luciana Lopes Fortes.
Data: | 2015-08-06
Ano: | 2015
Palavras-chave: | Biotecnologia; Botânica epiphytic orchid; In vitro propagation; Protocorms; Protocorm-like body 2.03.00.00-0.
Resumo: | An efficient in vitro propagation method was established for Brasilidium forbesii (Hook.) Campacci using transverse and longitudinal thin cell layer (tTCL and lTCL, respectively) culture systems. Six-month-old protocorms from in vitro germinated seeds were used for this study. TCLs (1.0-mm thick) from protocorms were grown on Woody Plant Medium (WPM) supplemented with benzyladenine (BA) (0.5–4.0 µM).The lTCL technique was more efficient for inducing protocorm-like bodies (PLBs) and regenerating shoots than the tTCL technique. The frequency of PLB formation was influenced by BA concentration, and the lTCL explant grown on a medium containing 2.0 µM BA produced the highest percentage of new protocorms (77%) with a total of 22.7 PLBs per explant, after the first subculture on the same medium. Plantlet development was optimal on WPM medium containing 3.0 g L-1 activated charcoal, and indole-3-butyric acid was not necessary for rooting. Regenerated plants were successfully acclimatized in a greenhouse after 16 weeks using vermiculite as the substrate (100% survival).
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês

Identificador:

http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/27276

10.4025/actascibiolsci.v37i2.27276

Editor: | Universidade Estadual De Maringá
Relação: | http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/27276/pdf_49
Formato: | application/pdf

Fonte:

Acta Scientiarum. Biological Sciences; v. 37, n. 2 (2015); 143-149

Acta Scientiarum. Biological Sciences; v. 37, n. 2 (2015); 143-149

1807-863X

1679-9283