Data/hora: 15/06/2024 17:59:39

Provedor de dados: | 3
País: | Brazil
Título: | Agave syrup as a substrate for inulinase production by Kluyveromyces marxianus NRRL Y-7571
Autores: | Oliveira, Luana Paula de Azevedo de; Oliveira, Tiago Felipe; Contiero, Jonas; Cazetta, Márcia Luciana.
Data: | 2016-12-08
Ano: | 2016
Palavras-chave: | 2.12.02.00-1 yeast; Microbial enzymes; Agricultural substrate Microbiologia aplicada.
Resumo: | The factorial planning was used to plan and optimize inulinase production by the yeast Kluyveromyces marxianus NRRL Y-7571. The experiments were conducted using a Central Composite Design (CCD) 22, at different concentrations of agave syrup (3.6 to 6.4%) and yeast extract (2.2 to 3.0%). After 96 hours of fermentation, the best condition for the inulinase production was 5% agave syrup and 2.5% yeast extract, which yielded an average of 129.21 U mL-1 of inulinase. Partial characterization of the crude enzyme showed that the optimal pH and temperature were 4.0 and 60°C, respectively. The enzyme showed thermal stability at 55°C for 4 hours.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês

Identificador:

http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/31489

10.4025/actascibiolsci.v38i3.31489

Editor: | Universidade Estadual De Maringá
Relação: | http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/31489/pdf
Formato: | application/pdf

Fonte:

Acta Scientiarum. Biological Sciences; v. 38, n. 3 (2016); 283-289

Acta Scientiarum. Biological Sciences; v. 38, n. 3 (2016); 283-289

1807-863X

1679-9283

Fonte: | Direitos autorais 2016 Acta Scientiarum. Biological Sciences