Data/hora: 11/06/2024 10:55:45

Provedor de dados: | 3
País: | Brazil
Título: | Structural and physical-chemical analyses of sulfated polysaccharides from the sea lettuce  Ulva lactuca and their effects on thrombin generation
Autores: | Barcellos, Priscila Gomes; Rodrigues, José Ariévilo Gurgel; Queiroz, Ismael Nilo Lino de; Araújo, Ianna Wivianne Fernandes de; Benevides, Norma Maria Barros; Mourão, Paulo Antônio de Souza.
Data: | 2018-11-30
Ano: | 2018
Palavras-chave: | Ulvales; Complex glycans; Chemical analysis; Thrombosis.
Resumo: | Ulva lactuca (Chlorophyceae) has biotechnologically-important sulfated-polysaccharides (Ul-SPs), but their potentials on thrombin generation (TG) are unknown. This study analyzed the structural and physical-chemical features of the Ul-SPs as modulators of TG. Proteolytic digestion yielded (13.13%) extract containing sulfate (20.43%) and total sugars (65.72%), besides ulvan consisting of rhamnose, xylose, glucose, glucuronic acid and α-/β-types glycosidic linkages as characterized by one-/two-dimensions nuclear magnetic resonance (NMR) experiments. Fractionation of the Ul-SPs by DEAE-cellulose chromatography yielded Ul-SP1 and Ul-SP2 (0.50 and 0.75 M NaCl, respectively) showing sulfation (15.72-18.04%) and total sugars (59.73-60.58%) consistent with the charge density pattern by combination of agarose/polyacrylamide gel eletrophoresis using sequential staining with toluidine blue and stains-all, although with slight differences in their sizes (40 and &gt;100 kDa, respectively). By both activated partial thromboplastin time (APTT) and prothrombin time (PT) tests, anticoagulation of the fractions was virtually detected by APTT (0.39 and 0.43 IU, respectively) against heparin (193 IU). Fractions acted differently on both intrinsic/extrinsic pathways in TG using 60-fold diluted human plasma, with 50% efficacies up to 8.3 µg, whereas at high concentrations suggested intrinsic hypercoagulability since heparin abolished both systems at low amounts. Ul-SPs block TG, but predicting thrombosis in increasing doses.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês

Identificador:

http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/34916

10.4025/actascibiolsci.v40i1.34916

Editor: | Universidade Estadual De Maringá

Relação:

http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/34916/pdf

http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/downloadSuppFile/34916/14062

Formato: | application/pdf

Fonte:

Acta Scientiarum. Biological Sciences; v. 40 (2018): Publicação Contínua; e34916

Acta Scientiarum. Biological Sciences; v. 40 (2018): Publicação Contínua; e34916

1807-863X

1679-9283

Fonte:

Direitos autorais 2018 Acta Scientiarum. Biological Sciences

http://creativecommons.org/licenses/by/4.0