Data/hora: 11/06/2024 12:03:29

Provedor de dados: | 3
País: | Brazil
Título: | In vitro inactivation of thrombin generation by polysulfated fractions isolated from the tropical coenocytic green seaweed Caulerpa racemosa (Caulerpaceae, Bryopsidales)
Autores: | Rodrigues, José Ariévilo Gurgel; Benevides, Norma Maria Barros; Tovar, Ana Maria Freire; Mourão, Paulo Antônio de Souza.
Data: | 2017-09-18
Ano: | 2017
Palavras-chave: | Chlorophyta; Complex glycans; Sulfation pattern; Clot.
Resumo: | Pharmacological efficacy of Caulerpa racemosa (Chlorophyta) sulfated polysaccharidic (SPs) fractions (F I→III) on models of coagulation and inflammation has been demonstrated, but not their effects on thrombin generation (TG). This study examined fractions for composition and physical-chemical characteristics and in vitro inactivation of TG by F I and F II in 60-fold diluted human plasma using continuous method. Papain-extraction yield of 0.7% revealed F I→III by DEAE-cellulose chromatography, with differences among the relative proportions of sulfate (17.37-24.00%), total sugars (30.03-48.34%) and absence of proteins. Charge density patterns and molecular sizes &gt; 100 kDa of the fractions were verified by both agarose/polyacrylamide analyses, respectively. These electrophoreses combined with toluidine blue/Stains-All also indicated nonSPs. Anticoagulant effects of 4.76 (F I), 12.00 (F II) and 2.32 (F II) IU mg-1 by activated partial thromboplastin time test were recorded against heparin (193 IU mg-1), without changes in prothrombin time. Diluted plasma treated with F I and F II reduced concentration-dependent and sulfation pattern TG by both intrinsic and extrinsic pathways, with 50% inactivation by intrinsic pathway of F II even at 4.1 µg. Heparin abolished TG at least 4-fold lower. Therefore, C. racemosa produces SPs with TG inhibition.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês

Identificador:

http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/32095

10.4025/actascibiolsci.v39i3.32095

Editor: | Universidade Estadual De Maringá
Relação: | http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/32095/pdf
Formato: | application/pdf

Fonte:

Acta Scientiarum. Biological Sciences; v. 39, n. 3 (2017); 283-292

Acta Scientiarum. Biological Sciences; v. 39, n. 3 (2017); 283-292

1807-863X

1679-9283

Fonte: | Direitos autorais 2017 Acta Scientiarum. Biological Sciences