Data/hora: 09/06/2024 17:59:57

Provedor de dados: | 3
País: | Brazil
Título: | Cell death induced by tamoxifen in human blood lymphocytes cultivated in vitro - doi: 10.4025/actascibiolsci.v32i4.7015
Autores: | Oliveira, Naila Francis Paulo de; UNICAMP; Genari, Selma Candelária; UNICAMP; Dolder, Heidi; UNICAMP.
Data: | 2010-11-03
Ano: | 2010
Palavras-chave: | 2.06.01.00-0 Apoptosis; Autophagy; Chemotherapeutic; Cytotoxicity; Morphology; Ultrastructure Citologia e Biologia Celular.
Resumo: | Many chemotherapeutic agents with a potential against solid tumors or leukemia can cause lymphopenia. Tamoxifen (TAM) is a synthetic non-steroidal anti-estrogen drug employed in female breast cancer treatment. The present study investigated the capacity of TAM to induce cell death in human lymphocytes cultivated in vitro. Lymphocytes were obtained from young (25-30 years; n = 3) and elderly women (58-77 years; n = 3) and cultivated for 24 or 48h, with or without TAM (20 &amp;micro;M). After the culture, cell viability, immunocytochemical response and ultrastructure were evaluated. TAM affected lymphocytes in a time- dependent manner, and cells obtained from elderly women were the most sensitive to TAM. Immunocytochemical analysis evidenced higher frequency of apoptosis in treated cells, and the ultrastructural study revealed autophagic vacuoles, differing from the controls. In summary, the treated lymphocytes were affected by TAM, leading to cell death by apoptosis and autophagy.
Idioma: | Inglês

Identificador:

http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/7015

10.4025/actascibiolsci.v32i4.7015

Editor: | Universidade Estadual De Maringá

Formato:

application/pdf

application/pdf

Fonte:

Acta Scientiarum. Biological Sciences; v. 32, n. 4 (2010); 415-421

Acta Scientiarum. Biological Sciences; v. 32, n. 4 (2010); 415-421

Fonte:

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DECLARAÇÃO DE ORIGINALIDADE E CESSÃO DE DIREITOS AUTORAIS  Declaro que o presente artigo é original, não tendo sido submetido à publicação em qualquer outro periódico nacional ou internacional, quer seja em parte ou em sua totalidade. Declaro, ainda, que uma vez publicado na revista Acta Scientiarum. Biological Sciences, editada pela Universidade Estadual de Maringá, o mesmo jamais será submetido por mim ou por qualquer um dos demais co-autores a qualquer outro meio de divulgação científica. Através deste instrumento, em meu nome e em nome dos demais co-autores, porventura existentes, cedo os direitos autorais do referido artigo à Universidade Estadual de Maringá e declaro estar ciente de que a não observância deste compromisso submeterá o infrator a sanções e penas previstas na Lei de Proteção de Direitos Autorias (Nº9609, de 19/02/98).