Data/hora: 09/06/2024 04:25:11

Provedor de dados: | 3
País: | Brazil
Título: | Enzyme expression in indica and japonica rice cultivars under saline stress - doi: 10.4025/actascibiolsci.v34i4.8535
Autores: | Lima, Maria da Graça de Souza; Universidade Federal de Pelotas; Lopes, Nei Fernandes; Universidade Federal de Pelotas; Zimmer, Paulo Dejalma; Universidade Federal de Pelotas; Meneghello, Geri Eduardo; Universidade Federal de Pelotas; Mendes, Cristina Rodrigues; Universidade Federal de Pelotas; Amarante, Luciano do; Universidade Federal de Pelotas.
Data: | 2012-09-04
Ano: | 2012
Palavras-chave: | 2.00.00.00-6; 2.03.03.00-9; 2.08.00.00-2 electrophoresis; Isoenzyme; Krebs cycle; O. sativa L.; Salinity Fisiologia Vegetal.
Resumo: | The southern State of Rio Grande do Sul (RS) is the main rice producer in Brazil with a 60% participation of the national production and 86% participation of the region. Rice culture irrigation system is done by flooding, which leads to soil salinization, a major environmental constraint to production since it alters the plants’ metabolism exposed to this type of stress. The indica cultivar, widely used in RS, has a higher sensitivity to salinity when compared to that of the japonica cultivar in other physiological aspects. Current research analyzes enzymes expression involved in salt-subjected indica and japonica rice cultivars’ respiration. Oryza sativa L. spp. japonica S.Kato (BRS Bojuru, IAS 12-9 Formosa and Goyakuman) and Oryza sativa L. spp. indica S. Kato (BRS Taim-7, BRS Atalanta and BRS Querencia) were the cultivars employed. Seedlings were transferred to 15 L basins containing 50% Hoagland nutrient solution increased by 0, 25, 50, 75 and 100 mM NaCl, and collected at 14, 28 and 42 days after transfer (DAT). Plant tissues were macerated and placed in eppendorf tubes with Scandálios extractor solution. Electrophoresis was performed in 7% of the polyacrylamide gels in vertical vats. Bands were revealed for the following enzymes systems: esterase, alcohol dehydrogenase, phosphoglucoisomerase, malate dehydrogenase, malic enzyme and alpha amylase. The enzymes expression was greater in subspecies japonica, with more intense bands in proportion to salinity increase. Results show that enzyme systems are involved in the salinity defense mechanisms in O. sativa spp. japonica cultivar.
Idioma: | Inglês

Identificador:

http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/8535

10.4025/actascibiolsci.v34i4.8535

Editor: | Universidade Estadual De Maringá

Formato:

application/pdf

application/pdf

Fonte:

Acta Scientiarum. Biological Sciences; v. 34, n. 4 (2012); 473-481

Acta Scientiarum. Biological Sciences; v. 34, n. 4 (2012); 473-481

Fonte:

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