Data/hora: 16/06/2024 00:01:51

Provedor de dados: | 3
País: | Brazil
Título: | Behavior of α-tomatine and tomatidine against several genera of trypanosomatids from insects and plants and Trypanosoma cruzi
Autores: | Evangelista, Adriane Feijó; Kavati, Erica Akemi; Jankevicius, José Vitor; Menolli, Rafael Andrade.
Data: | 2018-11-30
Ano: | 2018
Palavras-chave: | 2.13.01.00-0 tomato alkaloids; Trypanocidal activity; Phytomonas; Leptomonas; Crithidia; Herpetomonas. Protozoologia.
Resumo: | Glycoalkaloids are important secondary metabolites accumulated by plants as protection against pathogens. One of them, α-tomatine, is found in high concentrations in green tomato fruits, while in the ripe fruits, its aglycone form, tomatidine, does not present a protective effect, and it is usual to find parasites of tomatoes like Phytomonas serpens in these ripe fruits. To investigate the sensitivity of trypanosomatids to the action of α-tomatine, we used logarithmic growth phase culture of 20 trypanosomatids from insects and plants and Trypanosoma cruzi. The lethal dose 50% (LD50) was determined by mixing 107 cells of the different isolates with α-tomatine at concentrations ranging from 10-3 to 10-8 M for 30 min at room temperature. The same tests performed with the tomatidine as a control showed no detectable toxicity against the same trypanosomatid cultures. The tests involved determination of the percentage (%) survival of the protozoan cultures in a Neubauer chamber using optical microscopy. The LD50 values varied from 10-4 to 10-6 M α-tomatine. Slight differences were detected among the LD50 values of the analyzed samples, and none of them showed evidence of resistance to the action of tomatinase, as shown by some pathogenic fungi.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês

Identificador:

http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/41853

10.4025/actascibiolsci.v40i1.41853

Editor: | Universidade Estadual De Maringá

Relação:

http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/41853/pdf

http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/downloadSuppFile/41853/17448

Formato: | application/pdf

Fonte:

Acta Scientiarum. Biological Sciences; v. 40 (2018): Publicação Contínua; e41853

Acta Scientiarum. Biological Sciences; v. 40 (2018): Publicação Contínua; e41853

1807-863X

1679-9283

Fonte:

Direitos autorais 2018 Acta Scientiarum. Biological Sciences

http://creativecommons.org/licenses/by/4.0