Data/hora: 06/06/2024 11:36:20

Provedor de dados: 	36	
País: 	Brazil	
Título: 	LD5o of the bacteria Aeromonas hydrophila to matrinxã, Brycon amazonicus	
Autores: 	Oliveira,Sarah Ragonha de; Souza,Rondon Tatsuta Yamane Baptista de; Brasil,Elenice Martins; Andrade,Jaqueline Inês Alves de; Nunes,Érica da Silva Santiago; Ono,Eduardo Akifumi; Affonso,Elizabeth Gusmão.	
Data: 	2011-01-01	
Ano: 	2011	
Palavras-chave: 	Aeromonas hydrophila; 96-h LD50; Matrinxã; Tropical fishes.	
Resumo: 	In order to determine the lethal dose (96-h LD50) of the bacteria Aeromonas hydrophila to matrinxã, Brycon amazonicus, to be applied in challenge tests, 90 fish (63.23 ± 6.39 g) were divided into five treatments, with different bacterial solutions: T1 - Control (0.9% NaCl saline solution); T2 (4 x 10(11) cells/ mL); T3 (5 x 10(11) cells/ mL); T4 (1.36 x 10(12) cells/ mL) and T5 (3.06 x 10(12) cells/ mL). Fish were previously anesthetized with benzocaine (60 mg L-1), inoculated in the peritoneal cavity with the bacterial suspensions and then distributed into fifteen 80-L test chambers, where the water variables were monitored and fish mortality was observed. The experiment was randomly designed in three replicates and the 96-h LD50 was estimated according to the trimmed Spearman-Karber method. Water quality variables remained within adequate ranges for fish health and performance. Fish mortality rate increased with the bacterial concentrations of A. hydrophila (T1 = 0%; T2 = 16.66%; T3 = 44.44%; T4 = 72.22% and T5 = 100%), and the first mortalities were observed after 57 h, although the signs of the bacterial infection were already observed 24 h after the inoculation. The results indicate that the 96-h LD50 value of A. hydrophila to matrinxã is 6.66 x 10(11) cells/ mL.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0044-59672011000200019	
Editor: 	Instituto Nacional de Pesquisas da Amazônia	
Relação: 	10.1590/S0044-59672011000200019	
Formato: 	text/html	
Fonte: 	Acta Amazonica  v.41 n.2 2011	
Fonte: 	info:eu-repo/semantics/openAccess