Data/hora: 01/06/2024 00:39:33

Provedor de dados: | 37
País: | Colombia
Título: | ANTIFUNGAL ACTIVITY OF NEEM (Azadirachta indica: MELIACEAE) EXTRACTS AGAINST DERMATOPHYTES
Autores: | OSPINA SALAZAR,Daniel Iván; HOYOS SÁNCHEZ,Rodrigo Alberto; OROZCO SÁNCHEZ,Fernando; ARANGO ARTEAGA,Myrtha; GÓMEZ LONDOÑO,Luisa Fernanda.
Data: | 2015-09-01
Ano: | 2015
Palavras-chave: | HPLC; Neem; Microbial sensitivity tests; Minimum inhibitory concentration; Terpenoids.
Resumo: | In order to assess the antifungal activity of methanolic extracts from neem tree (Azadirachta indica A. Juss.), several bioassays were conducted following M38-A2 broth microdilution method on 14 isolates of the dermatophytes Trichophytonmentagrophytes, Trichophyton rubrum, Microsporum canis and Epidermophyton floccosum. Neem extracts were obtained through methanol-hexane partitioning of mature green leaves and seed oil. Furthermore, high performance liquid chromatography (HPLC) analyses were carried out to relate the chemical profile with their content of terpenoids, ofwidely known antifungal activity. The antimycotic Terbinafine served as a positive control. Results showed that there was total growth inhibition of the dermatophytes isolates at minimal inhibitory concentrations (MIC) between 50 μg/mL and 200 μg/mL for leaves extract, and between 625 μg/mL and 2500 μg/mL for seed oil extract. The MIC of positive control (Terbinafine) ranged between 0.0019 μg/mL and 0.0313 μg/mL. Both neem leaves and seed oil methanol extracts exhibited different chromatographic profiles by HPLC, which could explain the differences observed in their antifungal activity. This analysis revealed the possible presence of terpenoids in both extracts, which are known to have biological activity. The results of this research are a new report on the therapeutic potential of neem to the control of dermatophytosis.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-548X2015000300017
Editor: | Universidad Nacional de Colombia, Facultad de Ciencias, Departamento de Biología
Relação: | 10.15446/abc.v20n3.45225
Formato: | text/html
Fonte: | Acta Biológica Colombiana  v.20 n.3 2015
Fonte: | info:eu-repo/semantics/openAccess