Data/hora: 16/06/2024 17:47:56

Provedor de dados: | 37
País: | Colombia
Título: | TRANSFORMING GROWTH FACTOR BETA HAS DUAL EFFECTS ON MMP9 AND uPA EXPRESSION IN HTR-8/SVneo HUMAN TROPHOBLASTIC CELL LINE
Autores: | NOVOA HERRÁN,Sandra Susana; CASTELBLANCO,Mariela; SÁNCHEZ -GÓMEZ,Myriam; UMAÑA PÉREZ,Adriana.
Data: | 2019-04-01
Ano: | 2019
Palavras-chave: | Computational biology; Matrix metalloproteinase; Placenta; Plasminogen; Polymerase chain reaction.
Resumo: | ABSTRACT Invasion of trophoblast into endometrium is vital for successful pregnancy development. MMP9 and uPA are key proteases in this process, but it is still not clear the regulation of its expression by Transforming Growth Factor Beta (TGF-β), a known negative regulator of trophoblast invasion. We evaluated the effect of TGF-β on the transcriptional expression of uPA and MMP9 over time, in HTR-8/SVneo trophoblast cells cultured with or without 0.5 % fetal bovine serum, via RT qPCR. The involved transcription factors and signaling pathways were analyzed in silico, using Proscan, Enrich, PCViz and Wiki Pathway. Results showed that TGF-β temporarily regulates the expression of uPA and MMP9. Serum modified the nature of TGF-β's effects on uPA expression, from negative without serum to positive with it, showing opposite effects on MMP9 expression. In silico analysis evidenced different transcription factors for each protease, some belonging to TGF-β signaling pathway, and crosstalk with MAPK and Wnt/β -catenin pathways. The TGF-β dual role is discussed proposing that serum affects the cellular context. Transcriptional regulation of MMP9 and uPA by TGF-β is differential and depends on serum presence and evaluation time.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-548X2019000100026
Editor: | Universidad Nacional de Colombia, Facultad de Ciencias, Departamento de Biología
Relação: | 10.15446/abc.v24n1.69527
Formato: | text/html
Fonte: | Acta Biológica Colombiana  v.24 n.1 2019
Fonte: | info:eu-repo/semantics/openAccess