Data/hora: 06/06/2024 11:48:19

Provedor de dados: | 39
País: | Brazil
Título: | Abiotic environmental conditions for germination and development of gametophytes of Cyathea phalerata Mart. (Cyatheaceae)
Autores: | Marcon,Catiuscia; Silveira,Tatieli; Schmitt,Jairo Lizandro; Droste,Annette.
Data: | 2017-03-01
Ano: | 2017
Palavras-chave: | Abiotic factors; Arborescent ferns; In vitro propagation; Reproduction; Spores.
Resumo: | ABSTRACT In order to successfully establish themselves in their natural environment, ferns need habitats with abiotic conditions that are suitable for spore germination and gametophyte development. The objective of this study was to assess the influence of abiotic factors on the initial development of Cyathea phalerata cultivated in vitro. Spore germination and gametophyte development were assessed under varying conditions of surface sterilization, pH, temperature and photoperiod. Exogenous contamination was eliminated by sterilizing spores with 2.5 % NaClO for 15 min and sowing them into a culture medium supplemented with nystatin. Spores germinated at all pHs tested. Gametophytic development was faster in acidic pHs. Cultures at 25 °C exhibited the highest percentages of germination and laminar gametophytes. The species produced its highest percentages of gametophytes in cultures with photoperiods between 6 and 18 h. The optimal abiotic conditions found here for in vitro development of C. phalerata are similar to those found in its natural habitat. The southern limit of this species to north of the 30th parallel in Rio Grande do Sul, Brazil, may be because further south spores do not encounter the ideal combined conditions of temperature, pH and photoperiod determined in the laboratory.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062017000100058
Editor: | Sociedade Botânica do Brasil
Relação: | 10.1590/0102-33062016abb0288
Formato: | text/html
Fonte: | Acta Botanica Brasilica  v.31 n.1 2017
Fonte: | info:eu-repo/semantics/openAccess