Data/hora: 06/06/2024 01:22:16

Provedor de dados: | 39
País: | Brazil
Título: | How to construct and use a simple device to prevent the formation of precipitates when using Sudan Black B for histology
Autores: | Oliveira,João Marcelo Santos de.
Data: | 2015-12-01
Ano: | 2015
Palavras-chave: | Histochemical tests; Histology; Lipids; Plant anatomy; Sudan Black B.
Resumo: | The present work aims to demonstrate the stages of fabrication and use of a simple device to avoid the formation or fixation of precipitates from Sudan Black B solution on tissues. The device consists of four coverslip fragments attached to a histology slide, which serve as points of support for the histological slide under analysis. To work properly, the histology slide with the sections should be placed with the sections facing downwards the device. A small space between the device and the histology slide is thereby created by the height of the coverslip fragments. When Sudan Black B is applied, the solution is maintained within the edges of the device and evaporation is minimized by the small space, thereby reducing the consequent formation of precipitates. Furthermore, by placing the sections facing downward the device, any sporadically formed precipitates are prevented from settling on and fixing to the sectioned tissues or organs. By avoiding the formation of precipitates, plant cells, tissues and organs can be better observed, diagnosed and photomicrographically recorded.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062015000400489
Editor: | Sociedade Botânica do Brasil
Relação: | 10.1590/0102-33062015abb0093
Formato: | text/html
Fonte: | Acta Botanica Brasilica  v.29 n.4 2015
Fonte: | info:eu-repo/semantics/openAccess