Data/hora: 02/06/2024 18:03:20

Provedor de dados: | 39
País: | Brazil
Título: | Separation of the genera in the subtribe Cassiinae (Leguminosae: Caesalpinioidae) using molecular markers
Autores: | Acharya,Laxmikanta; Mukherjee,Arup Kumar; Panda,Pratap Chandra.
Data: | 2011-03-01
Ano: | 2011
Palavras-chave: | AFLP; Cassia; ISSR; Molecular phylogeny; RAPD.
Resumo: | Random amplified polymorphic DNA (RAPD), Inter simple sequence repeat (ISSR) and Amplified fragment length polymorphism (AFLP) markers were used to verify the segregation of the genus Cassia L. senso lato into three distinct genera namely Chamaecrista Moench., Senna P. Mill. and Cassia L. sensostricto Eighteen representatives of the three taxa were characterized using the molecular markers. 25 RAPD, six ISSR primers and six AFLP primer combinations resulted in the amplification of 612, 115 and 622 bands (loci) respectively. Most of the loci are found to be polymorphic, showing high degrees of genetic diversity among the different taxa studied. The dendrogram constructed on the basis of the RAPD, ISSR and AFLP data using SHAN clustering, divided Cassia L. senso lato. into three different clusters as Chamaecrista Moench. Senna P. Mill. and Cassia L. senso stricto High bootstrap value revealed that all the clusters were stable and robust. It was observed from the present investigation that these genera have their identity at molecular level, which supports the elevation of the genus Cassia L. senso lato to the level of subtribe Cassiinae and segregation into three distinct genera instead of intrageneric categories.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062011000100026
Editor: | Sociedade Botânica do Brasil
Relação: | 10.1590/S0102-33062011000100026
Formato: | text/html
Fonte: | Acta Botanica Brasilica  v.25 n.1 2011
Fonte: | info:eu-repo/semantics/openAccess