Data/hora: 17/06/2024 02:40:53

Provedor de dados: 	42	
País: 	Brazil	
Título: 	Evaluation of morpho-anatomical and chemical differences between varieties of the medicinal plant Casearia sylvestris Swartz	
Autores: 	CLAUDINO,JOSIANE C.; SACRAMENTO,LUIS V.S. DO; KOCH,INGRID; SANTOS,HELEN A.; CAVALHEIRO,ALBERTO J.; TININIS,ARISTEU G.; SANTOS,ANDRé G. DOS.	
Data: 	2013-01-01	
Ano: 	2013	
Palavras-chave: 	Casearia sylvestris; Chromatography; Diterpenes; Morpho-anatomy.	
Resumo: 	Casearia sylvestris Swartz (Salicaceae) has been used in traditional medicine and its leaf extracts have been exhibited important pharmacological activities. The species presents morphological, chemical and genetic variation. Two varieties are considered due external morphological differences: C. sylvestris var. sylvestris and var. lingua. There are difficulties in definition of these varieties. The objective of this work is to evaluate chemical and morpho-anatomical differences between C. sylvestris varieties that can be applied in their distinction for pharmaceutical or botanical purposes. Transverse and paradermic sections of leaves were prepared for morpho-anatomical, histochemical and quantitative microscopy (stomatal and palisade index) analyses. Diterpene profiles of the specimens were obtained by HPLC-DAD and TLC. Morpho-anatomical analyses demonstrated significant differences between the varieties only in paradermic sections: var.sylvestris - polygonal epidermic cell walls and hypostomatic; var. lingua - rounded epidermic cell walls and amphistomatic. No differences were observed for stomatal index; palisade index was found 2.8 for var. lingua and 3.9 for var.sylvestris. Chromatographic analyses confirmed previous results demonstrating that diterpene profile in varieties differs, with predominance of these metabolites in var. sylvestris. In conclusion, this work indicates that chromatographic analysis besides morpho-anatomical analysis can be applied in distinction of C. sylvestris varieties.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652013000401253	
Editor: 	Academia Brasileira de Ciências	
Relação: 	10.1590/0001-3765201393812	
Formato: 	text/html	
Fonte: 	Anais da Academia Brasileira de Ciências  v.85 n.4 2013	
Fonte: 	info:eu-repo/semantics/openAccess