Data/hora: 09/06/2024 14:35:14

Provedor de dados: | 42
País: | Brazil
Título: | Biochemical characterization of the nuclease StoNurA from the hyperthermophilic archaeon Sulfolobus tokodaii
Autores: | Wei,Tao; Yang,Kunpeng; Zang,Jie; Mao,Duobin.
Data: | 2018-09-01
Ano: | 2018
Palavras-chave: | DNA nuclease; StoNurA; Dimer; Interaction; Recombinational repair.
Resumo: | Abstract The DNA nuclease gene ST2109 has been cloned from the hyperthermophilic archaeon Sulfolobus tokodaii and expressed in Escherichia coli. The recombinant protein StoNurA has been purified to homogeneity by affinity chromatography and gel filtration chromatography. Biochemical analyses demonstrated that StoNurA exhibited DNA binding and 5’–3’ exonuclease activities towards ssDNA and dsDNA. The temperature and pH optima of StoNurA were determined to be 65 °C and 8.0, respectively. The activity of StoNurA was found to be dependent of Mn2+, and its half-life of heat inactivation at 100 °C was 5 min. Gel filtration chromatography revealed that StoNurA could form dimers in solution. Pull-down assays also showed that StoNurA physically interacted with a DNA helicase (StoHerA). Our data suggest that NurA may play a key functional role in the processing of DNA recombinational repair.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652018000602731
Editor: | Academia Brasileira de Ciências
Relação: | 10.1590/0001-3765201820160031
Formato: | text/html
Fonte: | Anais da Academia Brasileira de Ciências  v.90 n.3 2018
Fonte: | info:eu-repo/semantics/openAccess