Data/hora: 14/06/2024 01:22:15

Provedor de dados: | 42
País: | Brazil
Título: | Cloning, expression, and analysis of the group 2 allergen from Dermatophagoides farinae from China
Autores: | Yu-bao,Cui; Zhou,Ying; Weihong,Shi; Guifang,Ma; Yang,Li; Yungang,Wang.
Data: | 2010-12-01
Ano: | 2010
Palavras-chave: | Dermatophagoides farinae; House-dust-mite allergy; Mite allergens; Der f 2; Allergen engineering; Immunotherapy; Bioinformatics.
Resumo: | To obtain the recombinant group 2 allergen product of Dermatophagoides farinae (Der f 2), the Der f 2 gene was synthesized by RT-PCR. The full-length cDNA comprised 441 nucleotides and was 99.3% identical to the reference sequence (GenBank AB195580). The cDNA was bound to vector pET28a to construct plasmid pET28a(+)-Der f 2, which was transformed into E. coli BL21 and induced by IPTG. SDS-PAGE showed a specific band of about 14kDa in the hole cell lysate. s estiated by chroatography, about 3.86 g of the recobinant product as obtained, which conjugated with serum IgE from asthmatic children. The protein had a signal peptide of 17 amino acids. Its secondary structure comprised an alpha helix (19.86%), an extended strand (30.82%), and a random coil (49.32%). The subcellular localization of this allergen was predicted to be at mitochondria. Furthermore, its function was shown to be associated with an MD-2-related lipid-recognition (ML) domain. The results of this study provide a solid foundation for large-scale production of the allergen for clinical diagnosis and treatent of allergic disorders.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652010000400017
Editor: | Academia Brasileira de Ciências
Relação: | 10.1590/S0001-37652010000400017
Formato: | text/html
Fonte: | Anais da Academia Brasileira de Ciências  v.82 n.4 2010
Fonte: | info:eu-repo/semantics/openAccess