Data/hora: 06/06/2024 01:37:57

Provedor de dados: 	42	
País: 	Brazil	
Título: 	Extracting DNA of nematodes communities from Argentine Pampas agricultural soils	
Autores: 	Mondino,Eduardo A.; Covacevich,Fernanda; Studdert,Guillermo A.; Pimentel,João P.; Berbara,Ricardo L.L.	
Data: 	2015-06-01	
Ano: 	2015	
Palavras-chave: 	Agroecosystems; Nematodes; Nucleic acid extraction; CTAB.	
Resumo: 	We examined four strategies (Tris/EDTA, sodium dodecyl sulfate, Chelex 100 resin and cetyltrimethylammonium bromide -CTAB-) for extracting nucleic acid (DNA) from communities of nematodes. Nematodes were isolated from an agricultural area under different management of long-term crop rotation experiment from Argentina during three seasons. After DNA extraction, Polymerase Chain Reaction-amplifications were performed and considered as indicators of successful DNA extraction. The CTAB combined with proteinase K and phenol-chloroform-isoamyl alcohol was the unique successful method because positive amplifications were obtained by using both eukaryotic and nematode specific primers. This work could contribute to biodiversity studies of nematodes on agroecosystems.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652015000200691	
Editor: 	Academia Brasileira de Ciências	
Relação: 	10.1590/0001-3765201520130110	
Formato: 	text/html	
Fonte: 	Anais da Academia Brasileira de Ciências  v.87 n.2 2015	
Fonte: 	info:eu-repo/semantics/openAccess