Data/hora: 06/06/2024 12:57:41

Provedor de dados: 	42	
País: 	Brazil	
Título: 	Magnetic Parkia pendula seed gum as matrix for Concanavalin A lectin immobilization and its application in affinity purification	
Autores: 	RÊGO,MOACYR J.B.M.; ALMEIDA,SINARA M.; BEZERRA,SÉRGIO A.; CARVALHO JÚNIOR,LUIZ B.; BELTRÃO,EDUARDO I.C.	
Data: 	2014-09-01	
Ano: 	2014	
Palavras-chave: 	Immobilization; Magnetization; Parkia pendula seed gum; Concanavalin A.	
Resumo: 	The present work aimed to magnetize Parkia pendula seeds gum and use it as a matrix for Concanavalin A covalent immobilization. This composite was applied in affinity purification of glycoconjugates. Parkia pendula seeds were hydrated and the gum provenient from the supernatant was precipitated and washed with ethanol and dried. The gum was magnetized in co-precipitation using solutions of Fe+2 and Fe+3. Matrix activation was accomplished with NaIO4. Magnetized Parkia pendula seeds gum with covalently immobilized Concanavalin A was used as an affinity matrix for the recognition of bovine serum fetuin glycoprotein. Fetuin elution was carried out with a solution of glucose (300mM) and evaluated through SDS-PAGE. The efficiency of lectin immobilization and fetuin purification were 63% and 14%, respectively. These results indicate that the composite produced is a promising magnetic polysaccharide matrix for lectins immobilization. Thus, such system can be applied for affinity purification allowing an easy recovery by magnetic field.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652014000301351	
Editor: 	Academia Brasileira de Ciências	
Relação: 	10.1590/0001-3765201420130316	
Formato: 	text/html	
Fonte: 	Anais da Academia Brasileira de Ciências  v.86 n.3 2014	
Fonte: 	info:eu-repo/semantics/openAccess