Data/hora: 06/06/2024 02:36:23

Provedor de dados: | 42
País: | Brazil
Título: | Oxidative stress responses of juvenile tambaqui Colossoma macropomum after short-term anesthesia with benzocaine and MS-222
Autores: | STRINGHETTA,GIOVANNA R.; BARBAS,LUIS A.L.; MALTEZ,LUCAS C.; SAMPAIO,LUÍS A.; MONSERRAT,JOSÉ M.; GARCIA,LUCIANO O.
Data: | 2017-01-01
Ano: | 2017
Palavras-chave: | Anesthetics; Fish; GST; Lipid peroxidation; Total antioxidant capacity.
Resumo: | ABSTRACT The present study aimed to evaluate the effects of benzocaine and tricaine methanesulfonate on oxidative stress parameters of juvenile tambaqui tissues. Fish (n=80) were anesthetized with benzocaine (100 mg L-1) or tricaine (240 mg L-1) and two control groups were used (non-anesthetized fish and fish exposed to ethanol-only). After anesthetic induction 10 fish/anesthetic were euthanized after 3, 12 and 24 hours post-anesthesia and tissue samplings (gills, liver and brain) were performed. Samples were submitted to analyses of enzyme activity glutathione-S-transferase (GST), cellular lipid peroxidation (TBARS) and total antioxidant capacity (ACAP). ACAP increased in gills of benzocaine treatment after 12 hours. The liver showed a reduction in ACAP of tricaine treatment after 12 hours. Both anesthetic treatments showed an increase of ACAP at 24 hours compared to control group. The activity of the GST enzyme increased in the gills for treatments benzocaine and tricaine after 3 and 12 hours. Liver showed increased GST activity (benzocaine after 24 hours and tricaine after 3 and 24 hours). Lipid damage decreased in gills (both anesthetics) and brain (tricaine) after 24 hours. The results demonstrate that benzocaine and tricaine did not cause oxidative damage in juvenile tambaqui under the experimental conditions herein established.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652017000502209
Editor: | Academia Brasileira de Ciências
Relação: | 10.1590/0001-3765201720160823
Formato: | text/html
Fonte: | Anais da Academia Brasileira de Ciências  v.89 n.3 suppl.0 2017
Fonte: | info:eu-repo/semantics/openAccess