Data/hora: 15/06/2024 12:38:56

Provedor de dados: 	42	
País: 	Brazil	
Título: 	Evaluation of protein extraction methods for enhanced proteomic analysis of tomato leaves and roots	
Autores: 	VILHENA,MILCA B.; FRANCO,MÔNICA R.; SCHMIDT,DAIANA; CARVALHO,GISELLE; AZEVEDO,RICARDO A.	
Data: 	2015-09-01	
Ano: 	2015	
Palavras-chave: 	Bidimensional electrophoresis; Proteins; Protocol optimization; Tomato.	
Resumo: 	Proteomics is an outstanding area in science whose increasing application has advanced to distinct purposes. A crucial aspect to achieve a good proteome resolution is the establishment of a methodology that results in the best quality and wide range representation of total proteins. Another important aspect is that in many studies, limited amounts of tissue and total protein in the tissue to be studied are found, making difficult the analysis. In order to test different parameters, combinations using minimum amount of tissue with 4 protocols for protein extraction from tomato (Solanum lycopersicum L.) leaves and roots were evaluated with special attention to their capacity for removing interferents and achieving suitable resolution in bidimensional gel electrophoresis, as well as satisfactory protein yield. Evaluation of the extraction protocols revealed large protein yield differences obtained for each one. TCA/acetone was shown to be the most efficient protocol, which allowed detection of 211 spots for leaves and 336 for roots using 500 µg of leaf protein and 800 µg of root protein per gel.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652015000401853	
Editor: 	Academia Brasileira de Ciências	
Relação: 	10.1590/0001-3765201520150116	
Formato: 	text/html	
Fonte: 	Anais da Academia Brasileira de Ciências  v.87 n.3 2015	
Fonte: 	info:eu-repo/semantics/openAccess