Data/hora: 05/06/2024 12:35:28

Provedor de dados: | 42
País: | Brazil
Título: | Multidrug resistance in tumour cells: characterisation of the multidrug resistant cell line K562-Lucena 1
Autores: | RUMJANEK,VIVIAN M.; TRINDADE,GILMA S.; WAGNER-SOUZA,KAREN; MELETTI-DE-OLIVEIRA,MICHELE C.; MARQUES-SANTOS,LUIS F.; MAIA,RAQUEL C.; CAPELLA,MÁRCIA A. M.
Data: | 2001-03-01
Ano: | 2001
Palavras-chave: | Multidrug resistance; Leukaemia; P-glycoprotein; Chemosensitisers.
Resumo: | Multidrug resistance to chemotherapy is a major obstacle in the treatment of cancer patients. The best characterised mechanism responsible for multidrug resistance involves the expression of the MDR-1 gene product, P-glycoprotein. However, the resistance process is multifactorial. Studies of multidrug resistance mechanisms have relied on the analysis of cancer cell lines that have been selected and present cross-reactivity to a broad range of anticancer agents. This work characterises a multidrug resistant cell line, originally selected for resistance to the Vinca alkaloid vincristine and derived from the human erythroleukaemia cell K562. This cell line, named Lucena 1, overexpresses P-glycoprotein and have its resistance reversed by the chemosensitisers verapamil, trifluoperazine and cyclosporins A, D and G. Furthermore, we demonstrated that methylene blue was capable of partially reversing the resistance in this cell line. On the contrary, the use of 5-fluorouracil increased the resistance of Lucena 1. In addition to chemotherapics, Lucena 1 cells were resistant to ultraviolet A radiation and hydrogen peroxide and failed to mobilise intracellular calcium when thapsigargin was used. Changes in the cytoskeleton of this cell line were also observed.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652001000100007
Editor: | Academia Brasileira de Ciências
Relação: | 10.1590/S0001-37652001000100007
Formato: | text/html
Fonte: | Anais da Academia Brasileira de Ciências  v.73 n.1 2001
Fonte: | info:eu-repo/semantics/openAccess