Data/hora: 09/06/2024 06:18:54

Provedor de dados: | 42
País: | Brazil
Título: | Altered hyaluronic acid content in tear fluid of patients with adenoviral conjunctivitis
Autores: | DREYFUSS,JULIANA L.; REGATIERI,CAIO V.; COELHO,BRUNO; BARBOSA,JOSÉ B.; FREITAS,DENISE DE; NADER,HELENA B.; MARTINS,JOÃO R.
Data: | 2015-03-01
Ano: | 2015
Palavras-chave: | Conjunctivitis; Glycosaminoglycans; Hyaluronic acid; Tear film.
Resumo: | The adenoviral conjunctivitis is one of the biggest causes of conjunctival infection in the world. Conjunctivitis causes relatively nonspecific symptoms, as hyperaemia and chemosis. Even after biomicroscopy, complex laboratory tests, such as viral culture, are necessary to identify the pathogen or its etiology. To contribute to the better understanding of the pathobiology of the adenoviral conjunctivitis, the tear fluids of patients with unilateral acute adenovirus conjunctivitis (UAAC), normal donors (control) and patients with allergic conjunctivitis were analyzed. Tear samples were collected with Schirmer strips from control, allergic conjunctivitis and UAAC patients, diagnosed by clinical signs. UAAC tears were tested positive in viral cultures. After the elution, HA was quantified using an ELISA-like fluorometric assay and the protein profile was determined by SDS-PAGE. A profound increase in the HA tear content in UAAC patients was found when compared to control and ALC. This HA increase in UAAC tears remarkably was not observed in tears from contralateral eyes without clinical signs, nor in allergic conjunctivitis. In addition a distinct profile of UAAC tear proteins was observed in patients with UAAC. The quantification of HA in the tear fluid is a rapid, sensitive and specific test. This molecule might be a biomarker candidate for acute conjunctivitis.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652015000100455
Editor: | Academia Brasileira de Ciências
Relação: | 10.1590/0001-3765201520140122
Formato: | text/html
Fonte: | Anais da Academia Brasileira de Ciências  v.87 n.1 2015
Fonte: | info:eu-repo/semantics/openAccess