Data/hora: 15/06/2024 08:01:24

Provedor de dados: 	42	
País: 	Brazil	
Título: 	Probiotics protect the intestinal wall of morphological changes caused by malnutrition	
Autores: 	AZEVEDO,JORGE F. DE; HERMES-ULIANA,CATCHIA; LIMA,DIRLENE P.; SANT'ANA,DÉBORA M.G.; ALVES,GILBERTO; ARAÚJO,EDUARDO J.A.	
Data: 	2014-09-01	
Ano: 	2014	
Palavras-chave: 	Bowel; Digestive system; Histology; Morphometry; Protein-energy malnutrition; Small intestine.	
Resumo: 	This study sought to morphometrically analyze the jejunal wall of protein-malnourished rats administered a probiotic supplement. The sample consisted of recently weaned Wistar rats (Rattus norvegicus) distributed among four groups: animals given a commercial diet (G1, n = 4); animals given the same ration as G1 plus a probiotic supplement (G2, n = 4); animals given a 4% protein diet (G3, n = 4); and animals given the same ration as G3 plus a probiotic supplement (G4, n = 4). After 12 weeks, part of the jejunum was harvested and subjected to routine histological processing. Transverse sections with a thickness of 3 µm were stained with HE, and histochemical techniques were used to assay for glycoconjugates, including staining with periodic acid-Schiff (PAS) + diastase, Alcian Blue (AB) solution at pH 2.5, and Alcian Blue solution at pH 1.0. Morphometric analysis of the bowel wall showed that the probiotic culture used in this study induced hypertrophy of several layers of the jejunal wall in well-nourished animals and reduced the bowel wall atrophy usually observed in protein-malnourished animals. Neither malnutrition nor the use of probiotics altered the relationship between the number of goblet cells and the number of enterocytes.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652014000301303	
Editor: 	Academia Brasileira de Ciências	
Relação: 	10.1590/0001-3765201420130224	
Formato: 	text/html	
Fonte: 	Anais da Academia Brasileira de Ciências  v.86 n.3 2014	
Fonte: 	info:eu-repo/semantics/openAccess