Data/hora: 02/06/2024 06:20:57

Provedor de dados: | 42
País: | Brazil
Título: | Are salty liquid food flavorings in vitro antitumor substances?
Autores: | CARVALHO,FRANCISCO R.S.; MOURA,ANTONIO G.; RODRIGUES,GARDENIA F.; NUNES,NARCIA M.F.; LIMA,DAISY J.B.; PESSOA,CLAUDIA; COSTA,MARCILIA P.; FERREIRA,PAULO M.P.; PERON,ANA PAULA.
Data: | 2016-09-01
Ano: | 2016
Palavras-chave: | Food additives; Allium cepa; Antiproliferative action; Neoplasic cells; Toxicity.
Resumo: | ABSTRACT The objective of this study was to evaluate the antiproliferative, cytotoxic and genotoxic potential of salty liquid synthetic flavorings of Butter, Cheddar Cheese and Onion. The antiproliferative potential (2.9-1500 µg/mL) was assessed by MTT assay after 72h using the human tumor lines SF-295 (glioblastoma), OVCAR-8 (ovarian), HCT-116 (colon) and HL-60 (promyelocytic leukemia) and primary cultures of murine Sarcoma 180 (S180) and peripheral blood mononuclear cells (PBMC). Allium cepa bulbs were exposed to growing respective doses (1 mL and 2 mL). Only Butter and Cheddar flavorings revealed cytotoxic activity on cancer cells, with IC50 values ranging from 125.4 µg/mL (Cheddar - HCT-116) to 402.6 µg/mL (Butter - OVCAR-8). Butter flavoring was the most cytotoxic on PBMC (136.3 µg/mL) and increased cell division rate in relation to the mitotic index but did not cause cellular aberrations. Onion and Cheddar flavorings reduced the mitotic index after 24h and 48h exposure, but only Onion flavoring resulted in cellular aberrations and mitotic spindle abnormalities, such as anaphase and telophase bridges, micronucleated cells, conchicine-metaphases and amplifications. So, Butter, Onion and/or Cheddar flavorings caused significant changes in the division of meristematic cells of A. cepa and presented cytotoxic action even on decontrolled proliferating human tumor cells.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652016000401419
Editor: | Academia Brasileira de Ciências
Relação: | 10.1590/0001-3765201620150553
Formato: | text/html
Fonte: | Anais da Academia Brasileira de Ciências  v.88 n.3 2016
Fonte: | info:eu-repo/semantics/openAccess