Data/hora: 06/06/2024 10:25:05

Provedor de dados: | 42
País: | Brazil
Título: | Development of a real-time PCR assay for the detection of the golden mussel ( Limnoperna fortunei , Mytilidae) in environmental samples
Autores: | PIE,MARCIO R.; STRÖHER,PATRÍCIA R.; AGOSTINIS,ANDRÉ O.; BELMONTE-LOPES,RICARDO; TADRA-SFEIR,MICHELLE Z.; OSTRENSKY,ANTONIO.
Data: | 2017-06-01
Ano: | 2017
Palavras-chave: | EDNA; Invasive species; Environmental DNA; Molecular identification.
Resumo: | ABSTRACT The golden mussel, Limnoperna fortunei, is among the most devastating invasive species in freshwater habitats worldwide, leading to severe environmental disturbances and economic losses. Therefore, management efforts would be greatly improved by methods that efficiently detect and quantify the abundance of the golden mussel in freshwater habitats, particularly in early stages of colonization. In this study, we describe a highly-sensitive real-time PCR assay targeting a 100-bp region of the COI mitochondrial gene of the golden mussel. The method was able to detect as little as 0.225 pg of target DNA. This assay represents an important contribution to surveillance methods, as well as to optimize field measures to contain and manage populations of the golden mussel in its introduced range.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652017000301041
Editor: | Academia Brasileira de Ciências
Relação: | 10.1590/0001-3765201720160723
Formato: | text/html
Fonte: | Anais da Academia Brasileira de Ciências  v.89 n.2 2017
Fonte: | info:eu-repo/semantics/openAccess