Data/hora: 13/06/2024 15:27:12

Provedor de dados: | 42
País: | Brazil
Título: | Somatic embryogenesis in the commercial papaya hybrid UENF/Caliman 01 relying on plantlet production from sexed adult hermaphrodite donor plants
Autores: | GOUVEA,DRIELLY S.; CHAGAS,KRISTHIANO; CIPRIANO,JAMILE L.D.; LOPES,JOSÉ CARLOS; SCHMILDT,EDILSON R.; OTONI,WAGNER C.; SCHMILDT,OMAR; ARAUJO,CAROLINE P. DE; ALEXANDRE,RODRIGO S.
Data: | 2019-01-01
Ano: | 2019
Palavras-chave: | Abscisic acid; Auxins; Carica papaya L.; Gibberellic acid; Hermaphrodite explants; Polyethylene glycol.
Resumo: | Abstract: Somatic embryogenesis from explants from hermaphrodite papaya mother plants is an alternative for the production of true-to-type plants without the need for sexing. This study aimed to analyze hormonal and osmotic inducers in different somatic embryogenesis stages in the commercial hermaphrodite hybrid papaya UENF/Caliman 01. Leaf disks from in vitro shoots originated from ex vitro hermaphrodite plants were cultured in induction medium supplemented with different concentrations of 2,4-D (6, 9, 12, 15, and 18 μM) and 4-CPA (19, 22, 25, 28, and 31 μM). After 90 days, the formation of somatic embryos was verified. The 2,4-D induced the formation of light brown calli with low frequency (20%) of somatic embryogenesis. However, 4-CPA (25 μM) induced 96% of embryogenic calli, which were transferred to maturation medium (MM) and cultured for 30 days. The MM contained ABA (0.5 μM) and AC (15 g L-1) and produced 36.6 somatic embryos callus-1, mainly on cotyledonary stage. Cotyledonary embryos were transferred to germination medium supplemented with gibberellic acid (GA3) (0.0, 1.44, 2.88, and 4.32 µM), and the conversion into plantlets was enhanced with GA3 at 2.88 µM.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652019000500856
Editor: | Academia Brasileira de Ciências
Relação: | 10.1590/0001-3765201920180504
Formato: | text/html
Fonte: | Anais da Academia Brasileira de Ciências  v.91 n.3 2019
Fonte: | info:eu-repo/semantics/openAccess