Data/hora: 07/06/2024 00:32:00

Provedor de dados: 	42	
País: 	Brazil	
Título: 	Identification of Listeria monocytogenes in cattle meat using biochemical methods and amplification of the hemolysin gene	
Autores: 	SILVA,ALESSANDRA S.; DUARTE,ELIZABETH A.A.; OLIVEIRA,THIAGO A.S. DE; EVANGELISTA-BARRETO,NORMA S.	
Data: 	2020-01-01	
Ano: 	2020	
Palavras-chave: 	Foodborne diseases; Listeriosis; Meat products; Hly A gene.	
Resumo: 	Abstract  In Brazil and in other countries of the world, studies have been conducted to identify Listeria monocytogenes in cattle meat that is preferably consumed undercooked and, when marketed without meeting strict phytosanitary requirements, may cause outbreaks of listeriosis. In the such, foodborne outbreaks, the methods used for the detection of the pathogen and the efficiency associated with them are crucial for the proper assessment. In this study, we used the techniques biochemical and molecular for identification of the L. monocytogenes isolated from 30 samples of the fresh beef, marketed in ten butchers’ shop of the free-fair from a municipality from the Bahia, Brazil. The results obtained from biochemical tests (catalase, motility, β-hemolysis and carbohydrate fermentation), as well as PCR analysis for the hly gene (hemolysin production is an important factor in the pathogenesis of listeriosis) revealed that 50% of butchers shops presented bovine meat contaminated with bacteria of the Listeria sp. and confirmed that 54.16% of the analyzed meat samples were positive for L. monocytogenes. This study highlights the importance of microbiological surveillance in free-fair to minimize the exposure of consumers to this foodborne pathogen.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652020000201004	
Editor: 	Academia Brasileira de Ciências	
Relação: 	10.1590/0001-3765202020180557	
Formato: 	text/html	
Fonte: 	Anais da Academia Brasileira de Ciências  v.92 suppl.1 2020	
Fonte: 	info:eu-repo/semantics/openAccess