Data/hora: 01/06/2024 06:14:00

Provedor de dados: | 42
País: | Brazil
Título: | Cryopreservation of mutton snapper (Lutjanus analis) sperm
Autores: | SANCHES,EDUARDO G.; OLIVEIRA,IDILI R.; SERRALHEIRO,PEDRO C. DA SILVA; CERQUEIRA,VINICIUS R.
Data: | 2013-09-01
Ano: | 2013
Palavras-chave: | Cryoprotector; Extender; Lutjanus analis; Reproduction; Sperm.
Resumo: | This study aimed to develop a protocol of semen cryopreservation of the mutton snapper Lutjanus analis. The interaction between three extenders ( pH 6.1; 7.8 and 8.2) , two concentrations of dimethyl sulfoxide ( DMSO, 5 and 10%) and three cooling rates ( -90; -60 and -30°C.min−1) on the sperm motility rate and motility time were analyzed by a factorial experiment. A sample of 30 fishes ( 1,261 ± 449 g) collected in the nature was kept in floating net cages. The semen was frozen by using cryogenic straws, in nitrogen vapour and transferred, later, to liquid nitrogen. Fertilization test was accomplished to evaluate the viability of the cryopreserved sperm. The highest sperm motility rate and motility time ( P &lt; 0.05) was achieved by combining extender C ( pH 8.2) with DMSO ( 10%) and cooling rate of -60°C.min−1 ( P &lt; 0.05) . The use of cryopreserved sperm presented fertilization rates higher than 59% validating the present protocol for mutton snapper.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652013000301083
Editor: | Academia Brasileira de Ciências
Relação: | 10.1590/S0001-37652013005000047
Formato: | text/html
Fonte: | Anais da Academia Brasileira de Ciências  v.85 n.3 2013
Fonte: | info:eu-repo/semantics/openAccess