Data/hora: 11/06/2024 04:10:12

Provedor de dados: 	42	
País: 	Brazil	
Título: 	In vitro antioxidant, DNA-damaged protection and antiproliferative activities of ethyl acetate and n-butanol extracts of Centaurea sphaerocephalaL.	
Autores: 	LAHNECHE,AMINA M.; BOUCHEHAM,RATIBA; OZEN,TEVFIK; ALTUN,MUHAMMED; BOUBEKRI,NASSIMA; DEMIRTAS,IBRAHIM; BICHA,SABRINA; BENTAMENE,ALI; BENAYACHE,FADILA; BENAYACHE,SAMIR; ZAMA,DJAMILA.	
Data: 	2019-01-01	
Ano: 	2019	
Palavras-chave: 	Centaurea sphaerocephala; Phenolics; Antioxidant; Lipid peroxidation; Antiproliferative; DNA-damaged inhibition.	
Resumo: 	Abstract: This study aimed to evaluate the in vitro antiproliferative and inhibition of oxidative DNA-damage activities of n-butanol (n-BuOH) extract of Centaurea sphaerocephala. The in vitro antioxidant activity of the ethyl acetate (EtOAc) and the n-BuOH extracts of this plant were also assayed. To investigate the antioxidant potential, extracts were tested for their capacity to scavenge 1,1-diphenyl-2-picrylhydrazyl (DPPH·) and to inhibit lipid peroxidation using the TBARs method. The contents of total phenolics and flavonoids were measured. Additionally, antiproliferative activity and DNA-damage inhibition of the n-BuOH extract was determined using XCELLigence RTCA instrument and photolyzing 46966 plasmid, respectively. The results exhibited that the scavenging abilities of the EtOAc extract were better than the n-BuOH extract with an IC50= 11.59 µg/mL and 16.67 µg/mL for both extracts, respectively. The phenolic and flavonoid contents were found higher in the n-BuOH and EtOAc extracts. Furthermore, our results showed that n-BuOH extract exhibited a remarkable inhibition of lipid peroxidation with an IC50 of 340.94±7.49 μg/mL and had an antiproliferative effect against Hela cells. Extracts of C. sphaerocephala showed antioxidant activity on scavenging DPPH·. In addition, the n-BuOH extract inhibited the lipid peroxidation and exhibited an antiproliferative effect against HeLa cells line (human cervix carcinoma).	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652019000500619	
Editor: 	Academia Brasileira de Ciências	
Relação: 	10.1590/0001-3765201920180462	
Formato: 	text/html	
Fonte: 	Anais da Academia Brasileira de Ciências  v.91 n.3 2019	
Fonte: 	info:eu-repo/semantics/openAccess