Data/hora: 10/06/2024 10:01:46

Provedor de dados: | 42
País: | Brazil
Título: | DNA damage in children and adolescents with cardiovascular disease risk factors
Autores: | Kliemann,Mariele; Prá,Daniel; Müller,Luiza L.; Hermes,Liziane; Horta,Jorge A.; Reckziegel,Miriam B.; Burgos,Miria S.; Maluf,Sharbel W.; Franke,Silvia I.R.; Silva,Juliana da.
Data: | 2012-09-01
Ano: | 2012
Palavras-chave: | Cardiovascular diseases; Micronucleus test; Comet assay; Diet; Anthropometry; Child; Adolescent.
Resumo: | The risk of developing cardiovascular disease (CVD) is related to lifestyle (e.g. diet, physical activity and smoking) as well as to genetic factors. This study aimed at evaluating the association between CVD risk factors and DNA damage levels in children and adolescents. Anthropometry, diet and serum CVD risk factors were evaluated by standard procedures. DNA damage levels were accessed by the comet assay (Single cell gel electrophoresis; SCGE) and cytokinesis-blocked micronucleus (CBMN) assays in leukocytes. A total of 34 children and adolescents selected from a population sample were divided into three groups according to their level of CVD risk. Moderate and high CVD risk subjects showed significantly higher body fat and serum CVD risk markers than low risk subjects (P&lt;0.05). High risk subjects also showed a significant increase in DNA damage, which was higher than that provided by low and moderate risk subjects according to SCGE, but not according to the CBMN assay. Vitamin C intake was inversely correlated with DNA damage by SCGE, and micronucleus (MN) was inversely correlated with folate intake. The present results indicate an increase in DNA damage that may be a consequence of oxidative stress in young individuals with risk factors for CVD, indicating that the DNA damage level can aid in evaluating the risk of CVD.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652012000300025
Editor: | Academia Brasileira de Ciências
Relação: | 10.1590/S0001-37652012005000039
Formato: | text/html
Fonte: | Anais da Academia Brasileira de Ciências  v.84 n.3 2012
Fonte: | info:eu-repo/semantics/openAccess