Data/hora: 15/06/2024 15:24:55

Provedor de dados: | 42
País: | Brazil
Título: | Genetic transformation of Brachiaria brizantha cv. Marandu by biolistics
Autores: | CABRAL,GLAUCIA B.; CARNEIRO,VERA T.C.; GOMES,ANA CRISTINA M.M.; LACERDA,ANA LUIZA; MARTINELLI,ADRIANA P.; DUSI,DIVA M.A.
Data: | 2018-04-01
Ano: | 2018
Palavras-chave: | Forage; Gus; HptII; Hygromycin.
Resumo: | ABSTRACT Brachiaria brizantha is a forage grass well adapted to tropical areas and cultivated in millions of hectares in Brazil. The apomictic mode of reproduction in this species, in addition to differences in ploidy between sexual and apomictic plants, impairs crossbreeding. The development of a methodology to transform apomictic cultivars will provide an option to introduce agronomic important traits to B. brizantha cv. Marandu. In addition, it will open the possibility to study in vivo the function of candidate genes involved in the apomictic reproduction. The objective of this work was to evaluate peeled seeds, isolated embryo from mature seeds, embryogenic calluses and embryogenic cell suspensions, as target explant for genetic transformation via biolistics. Plasmids bearing the marker genes gus and hptII under the control of the rice actin 1 promoter (pAct1-Os) or the maize ubiquitin 1 promoter (pUbi1Zm) were used. All the target-explants used were suitable for transient gene expression after bombardment, showing gus expression and resistance to hygromycin. Using embryogenic calluses and cell suspensions as target tissues, transgenic plants were regenerated and transgenes detected.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652018000401789
Editor: | Academia Brasileira de Ciências
Relação: | 10.1590/0001-3765201820170842
Formato: | text/html
Fonte: | Anais da Academia Brasileira de Ciências  v.90 n.2 2018
Fonte: | info:eu-repo/semantics/openAccess