Data/hora: 03/06/2024 10:12:33

Provedor de dados: | 42
País: | Brazil
Título: | Study of the antiproliferative potential of seed extracts from Northeastern Brazilian plants
Autores: | Ferreira,Paulo Michel P.; Farias,Davi F.; Viana,Martônio P.; Souza,Terezinha M.; Vasconcelos,Ilka M.; Soares,Bruno M.; Pessoa,Cláudia; Costa-Lotufo,Letícia V.; Moraes,Manoel O.; Carvalho,Ana F.U.
Data: | 2011-09-01
Ano: | 2011
Palavras-chave: | Antiproliferative potential; Northeastern Brazilian plants; Myracrodruon urundeuva; Sarcoa 180 tumor; Seed extracts.
Resumo: | This study assessed the antiproliferative and cytotoxic potential against tumor lines of ethanolic seed extracts of 21 plant species belonging to different families from Northeastern Brazil. In addition, some underlying mechanisms involved in this cytotoxicity were also investigated. Among the 21 extracts tested, the MTT assay after 72 h of incubation demonstrated that only the ethanolic extract obtained from Myracrodruon urundeuva seeds (EEMUS), which has steroids, alkaloids and phenols, showed in vitro cytotoxic activity against human cancer cells, being 2-fold more active on leukemia HL-60 line [IC50 value of 12.5 (9.5-16.7) μg/mL] than on glioblastoma SF-295 [IC50 of 25.1 (17.3-36.3) μg/mL] and Sarcoma 180 cells [IC50 of 38.1 (33.5-43.4) μg/mL]. After 72h exposure, flow cytometric and morphological analyses of HL-60-treated cells showed that EEMUS caused decrease in cell number, volume and viability as well as internucleosomal DNA fragmentation in a dose-dependent way, suggesting that the EEMUS triggers apoptotic pathways of cell death.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652011000300023
Editor: | Academia Brasileira de Ciências
Relação: | 10.1590/S0001-37652011005000017
Formato: | text/html
Fonte: | Anais da Academia Brasileira de Ciências  v.83 n.3 2011
Fonte: | info:eu-repo/semantics/openAccess