Data/hora: 02/06/2024 17:29:18

Provedor de dados: | 44
País: | Brazil
Título: | RAPD analysis revealing polymorphism in egg parasitoids of soybean stink bugs (Hemiptera: Pentatomidae)
Autores: | Aljanabi,Salah M.; Loiácono,Marta S.; Lourenço,Rodrigo T.; Borges,Miguel; Tigano,Myrian S.
Data: | 1998-09-01
Ano: | 1998
Palavras-chave: | Insecta; Microhymenoptera parasitoids; Genetic variability; Molecular markers; RAPD.
Resumo: | The solitary egg parasitoids (Hymenoptera: Scelionidae) of Pentatomidae species complex are potential candidates for soybean (Glycine max L.) integrated pest management programs in Brazil. The correct identification of species and strains of these parasitoids is necessary to implement the use of biological control. Polymerase chain reaction (PCR) was used to generate random amplification of polymorphic DNA (RAPD) from male individuals of Telenomus podisi Ashmead and Trissolcus spp. collected in a soybean field in Brasília, Brazil in 1996. The analysis of 18 primers used to screen Trissolcus spp. individuals revealed different patterns related to the species analyzed. These results indicate the potential of RAPD markers to differentiate T. teretis Johnson, T. urichi Crawford and T. basalis (Wollaston). The 13 selected primers used to analyze T. podisi individuals produced 103 clear polymorphic scorable markers. Different genotypes were observed within this population analyzed, although the cluster analysis applied to the RAPD data showed high homogeneity (similarity &gt;78.2%) among individuals.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0301-80591998000300009
Editor: | Sociedade Entomológica do Brasil
Relação: | 10.1590/S0301-80591998000300009
Formato: | text/html
Fonte: | Anais da Sociedade Entomológica do Brasil  v.27 n.3 1998
Fonte: | info:eu-repo/semantics/openAccess