Data/hora: 11/06/2024 11:56:04

Provedor de dados: | 45
País: | Brazil
Título: | (+)-Catechin and (-)-epigallocatechin gallate: are these promising antioxidant therapies for frozen goat semen?
Autores: | Silva,E.C.B.; Arruda,L.C.P.; Vieira,J.I.T.; Soares,P.C.; Guerra,M.M.P.
Data: | 2019-04-01
Ano: | 2019
Palavras-chave: | Antioxidants; Cryopreservation; Flavonoids; Oxidative stress; Semen.
Resumo: | ABSTRACT The aim of this study was to evaluate the effects of different concentrations of (+)-catechin or (-)-epigallocatechin gallate (EGCG) on goat semen freezability. Poolsof semen were processed (Experiment 1: 0, 15, 25, 50, 75, or 100µM (+)-catechin; Experiment 2: 0, 15, 25, 50, 75, or 100µM EGCG) and frozen. After thawing, the samples were evaluated for kinematics, plasma membrane (PMi) and acrosome integrity, morphology, and oxidative stress, at 0 and 1h. In Experiment 1, at 0h, VSL and VAP were greater (P&lt;0.05) with 15µM than with 50 and 100; WOB was lower (P&lt;0.05) with 100µM than with 0, 15, and 25; and BCF was higher (P&lt;0.05) with 75 and 100µM than with 0. In turn, in Experiment 2, progressive motility was higher (P&lt;0.05) with0 and 15µM than with50 and 75; LIN was lower (P&lt;0.05) with75 and100µM than with0 and 15; WOB was higher (P&lt;0.05) with0 and 15µM; and PMi was greater (P&lt;0.05) with100µM than 0. Thus, (+)-catechin or EGCG at higher concentrations inhibits the kinematics of frozen goat sperm, in a transitory way, and 100µM of EGCG preserves the PMi.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352019000200521
Editor: | Universidade Federal de Minas Gerais, Escola de Veterinária
Relação: | 10.1590/1678-4162-10539
Formato: | text/html
Fonte: | Arquivo Brasileiro de Medicina Veterinária e Zootecnia  v.71 n.2 2019
Fonte: | info:eu-repo/semantics/openAccess