Data/hora: 09/06/2024 04:02:21

Provedor de dados: | 45
País: | Brazil
Título: | ELISA and modified toxin-binding inhibition test for quality control of the clostridial vaccine processes
Autores: | Sobrinho,E.M.; Almeida,A.C.; Brandi,I.V.; Colen,F.; Lobato,F.C.F.; Cangussu,A.S.R.; Santos,H.O.; Quintilio,W.; Sari,R.S.
Data: | 2014-06-01
Ano: | 2014
Palavras-chave: | Epsilon toxicoid; Clostridiosis; In vitro alternative method.
Resumo: | This study aimed to assess and standardize the ELISA and modified ToBI test in vitro methods in order to verify the potency of epsilon toxicoid in comparison with the in vivo TCP method. The following epsilon toxoids were used: NIBSC standard from batches 375/07, 532/08, 551/08, 373/07 and 378/07. These were evaluated using a TCP test, ELISA and ToBI tests. The results indicate that the correlation ratio between the dilutions of standard NIBSC toxicoid and absorbance values of 89.44% obtained with the ELISA method support the use of the curve to evaluate epsilon toxoids. However, it was observed that the absorbance values were similar for all toxoids, thus presenting no significant difference between higher and lower concentration toxoids. For the ToBI test, the correlation ratio of 96.76, obtained in the curve pattern, demonstrates the effectiveness of the curve to be used in the epsilon toxoid evaluation. The correlation ratio between the titration degrees of toxoids obtained through TCP and ToBI tests was higher than 90%. It is concluded that the type of ELISA test used does present discriminative power for toxoids with different concentrations, which does not support the use of this technique for such a purpose. The ToBI test can be used as a screening method for it is sensitive and effective to detect epsilon toxicoid produced by C. perfringens type D.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352014000300713
Editor: | Universidade Federal de Minas Gerais, Escola de Veterinária
Relação: | 10.1590/1678-41625407
Formato: | text/html
Fonte: | Arquivo Brasileiro de Medicina Veterinária e Zootecnia  v.66 n.3 2014
Fonte: | info:eu-repo/semantics/openAccess