Data/hora: 07/06/2024 08:37:42

Provedor de dados: | 45
País: | Brazil
Título: | Characterization of mesenchymal stem cells derived from equine adipose tissue
Autores: | Carvalho,A.M.; Yamada,A.L.M.; Golim,M.A.; Álvarez,L.E.C.; Jorge,L.L.; Conceição,M.L.; Deffune,E.; Hussni,C.A.; Alves,A.L.G.
Data: | 2013-08-01
Ano: | 2013
Palavras-chave: | Immunophenotypic characterization; Differentiation; Equine; Adipose tissue; Mesenchymal stem cell.
Resumo: | Stem cell therapy has shown promising results in tendinitis and osteoarthritis in equine medicine. The purpose of this work was to characterize the adipose-derived mesenchymal stem cells (AdMSCs) in horses through (1) the assessment of the capacity of progenitor cells to perform adipogenic, osteogenic and chondrogenic differentiation; and (2) flow cytometry analysis using the stemness related markers: CD44, CD90, CD105 and MHC Class II. Five mixed-breed horses, aged 2-4 years-old were used to collect adipose tissue from the base of the tail. After isolation and culture of AdMSCs, immunophenotypic characterization was performed through flow cytometry. There was a high expression of CD44, CD90 and CD105, and no expression of MHC Class II markers. The tri-lineage differentiation was confirmed by specific staining: adipogenic (Oil Red O), osteogenic (Alizarin Red), and chondrogenic (Alcian Blue). The equine AdMSCs are a promising type of adult progenitor cell for tissue engineering in veterinary medicine.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352013000400001
Editor: | Universidade Federal de Minas Gerais, Escola de Veterinária
Relação: | 10.1590/S0102-09352013000400001
Formato: | text/html
Fonte: | Arquivo Brasileiro de Medicina Veterinária e Zootecnia  v.65 n.4 2013
Fonte: | info:eu-repo/semantics/openAccess