Data/hora: 01/06/2024 10:33:36

Provedor de dados: | 45
País: | Brazil
Título: | Comparison between different dilution rates on canine semen freezing using Tris-buffer with the addition of egg-yolk and glycerol
Autores: | Silva,A.R.; Cardoso,R.C.S.; Silva,L.D.M.
Data: | 2005-12-01
Ano: | 2005
Palavras-chave: | Dog; Semen; Extender; Freezing; Dilution.
Resumo: | Standardized sperm concentration and volume:volume extension were compared as dilution rates for canine semen freezing. Six proven stud dogs were submitted to two seminal collections by manual stimulation. Semen was evaluated and extended in tris plus egg-yolk and glycerol according to two different dilution rates. The first one was based on a standardized sperm concentration of 200x10(6) spermatozoa/ml and the second was a volume:volume extension at a proportion of one part semen to one part extender. Semen was frozen, stored in liquid nitrogen and thawed after one week. Sperm motility and vigor were appraised after each stage of the process and at 15 and 30min post-thawing. Sperm morphology was analyzed after collection and thawing. No differences were observed between treatments after thawing regarding sperm motility and vigor, normal sperm morphology rate or longevity. Both dilution rates can be efficiently used for canine semen freezing.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352005000600010
Editor: | Universidade Federal de Minas Gerais, Escola de Veterinária
Relação: | 10.1590/S0102-09352005000600010
Formato: | text/html
Fonte: | Arquivo Brasileiro de Medicina Veterinária e Zootecnia  v.57 n.6 2005
Fonte: | info:eu-repo/semantics/openAccess