Data/hora: 05/06/2024 01:11:21

Provedor de dados: | 45
País: | Brazil
Título: | Development and standardization of a western blotting test for detection of antibodies against B. abortus
Autores: | Falcão,M.V.D.; Santana,V.L.A.; Corrêa,F.N.; Tenório,J.A. B.; Mota,R.A.
Data: | 2019-02-01
Ano: | 2019
Palavras-chave: | Brucellosis; Immunodiagnosis; Proteomics; Serology.
Resumo: | ABSTRACT Brucellosis is an infectious disease caused by bacteria of the genus Brucella spp. with diagnosis based on use of serological techniques. The present study aimed to develop and standardize a western blotting (WB) test for detection of antibodies against B. abortus. Samples from two groups of cattle were analyzed: group I: 60 serum samples from true positive and true negative vaccinated animals (30 positive samples from infected animals according to rose bengal test (RBT), 2-mercaptoethanol, serum agglutination test (SAT) and complement fixation test (CFT) and 30 RBT negatives samples); group II: 383 field samples (90 positive and 293 CFT negative sera). The most reactive band in the western blotting, which properly identified and separated infected from non - infected had a molecular weight of ≤ 20kDa. The sensitivity, specificity and accuracy of the WB compared to RBT was 93%, 99%, 98%, respectively and k= 0.938. When compared to CFT, the sensitivity, specificity and accuracy of the WB was 97%, 98% and 97%, respectively and k= 0.929. The WB developed and standardized in the present study is a serological test with potential use as a confirmatory test for the diagnosis of bovine brucellosis.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352019000100160
Editor: | Universidade Federal de Minas Gerais, Escola de Veterinária
Relação: | 10.1590/1678-4162-10290
Formato: | text/html
Fonte: | Arquivo Brasileiro de Medicina Veterinária e Zootecnia  v.71 n.1 2019
Fonte: | info:eu-repo/semantics/openAccess