Data/hora: 01/06/2024 01:27:21

Provedor de dados: | 45
País: | Brazil
Título: | Passive immunity in cattle against enterotoxigenic Escherichia coli: serologic evaluation of a bacterin containing K99 and F41 fimbriae in colostrum of vaccinated females and calf serum
Autores: | Figueiredo,H.C.P.; Lage,A.P.; Pereira Júnior,F.N.; Leite,R.C.
Data: | 2004-08-01
Ano: | 2004
Palavras-chave: | Calf; Enterotoxigenic Escherichia coli; Vaccine; Diarrhea; K99; F41.
Resumo: | A bacterin from enterotoxigenic Escherichia coli (ETEC), containing fimbriae K99 and F41, was produced and its capacity to induce anti-K99 and anti-F41 antibodies in colostrum of vaccinated cows and in calf serum, and the persistence of these antibodies in neonates were determined. Three experiments were performed on two commercial farms. In all experiments animals were allotted randomly to the blocks, each block consisting of two pregnant females (a vaccinated one and a control one) and their respective calves. In experiment A (farm 1), comprised of 18 blocks, the animals received a vaccine dose 30 days before delivery. In experiment B (farm 1), consisted of 26 blocks, the animals received two vaccine doses (60 and 30 days before delivery). In experiment C (farm 2), consisted of 22 blocks, the animals received two vaccine doses (60 and 30 days before delivery). In experiments A and B pregnant cows and heifers were used and colostrum and serum from 24- to 36-hour-old calves were collected. In experiment C, pregnant embryo-recipient heifers were used and colostrum and sera from calves at 7, 14, 28 and 42 days of age were collected. Anti-K99 and anti-F41 antibodies were detected by ELISA using purified K99 and F41 fimbrial antigens. In experiment A no difference between treated and control groups was observed for the concentration of anti-K99 and anti-F41 antibodies in colostrum and calf serum. In experiment B a difference (P&lt;0.001) was observed for colostrum of vaccinated females and for serum of their calves. In experiment C, difference between vaccinated and control animals was observed for colostrum and calf serum at 7, 14, 28 (P&lt;0.001 in all cases) and 42 days of age (P= 0.003). The results showed the efficiency of the bacterin to induce detectable humoral immune response.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352004000400001
Editor: | Universidade Federal de Minas Gerais, Escola de Veterinária
Relação: | 10.1590/S0102-09352004000400001
Formato: | text/html
Fonte: | Arquivo Brasileiro de Medicina Veterinária e Zootecnia  v.56 n.4 2004
Fonte: | info:eu-repo/semantics/openAccess