Data/hora: 16/06/2024 00:28:44

Provedor de dados: | 45
País: | Brazil
Título: | Avaliação da urina e de leucócitos como amostras biológicas para a detecção ante mortem do vírus da cinomose canina por RT-PCR em cães naturalmente infectados
Autores: | Negrão,F.J.; Alfieri,A.A.; Alfieri,A.F.
Data: | 2007-02-01
Ano: | 2007
Palavras-chave: | Cão; Cinomose; Diagnóstico; RT-PCR.
Resumo: | Urine and leucocytes were comparatively evaluated as clinical samples for ante mortem detection of the canine distemper virus (CDV) by a reverse transcription-polymerase chain reaction (RT-PCR) assay. One hundred and eighty eight dogs with clinical symptoms of distemper, were distributed in three groups. The group A was constituted of 93 dogs with systemic signs of distemper; the group B by 11 dogs with neurological signs, and the group C by 84 dogs that presented simultaneously systemic and neurological signs. In 66.5% (125/188) of the dogs was amplified an amplicon with 287 base pair of the CDV nucleoprotein gene. In 60.8% (76/125) of the animals the CDV was detected simultaneously in the urine and leucocytes, and in 39.2% (49/125) of the dogs just a type of clinical sample (urine: n=37; leucocytes: n=12) was positive. These results demonstrate that the different forms of clinical distemper disease can hinder the choice of only one type of clinical sample to carry out the ante mortem etiological diagnosis of CDV infection, and false-negative results can be generated.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Português
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352007000100042
Editor: | Universidade Federal de Minas Gerais, Escola de Veterinária
Relação: | 10.1590/S0102-09352007000100042
Formato: | text/html
Fonte: | Arquivo Brasileiro de Medicina Veterinária e Zootecnia  v.59 n.1 2007
Fonte: | info:eu-repo/semantics/openAccess