Data/hora: 02/06/2024 09:38:22

Provedor de dados: | 45
País: | Brazil
Título: | ELISA indireto para detecção de IgG antivírus da doença de Newcastle em soro de codorna
Autores: | Oliveira,D.D.; Folgueras-Flatschart,A.V.; Flatschart,R.B.; Resende,J.S.; Abreu,J.T.; Martins,N.R.S.
Data: | 2007-10-01
Ano: | 2007
Palavras-chave: | Codorna japonesa; ELISA; IgG; Doença de Newcastle.
Resumo: | An indirect ELISA for the detection of japanese quail IgG specific to Newcastle disease virus (NDV) was developed. The secondary anti-quail IgG was produced in Balb/c mice, by inoculating Freund's complete adjuvant emulsified japanese quail-IgG extract. The purification of IgG was achieved using the caprilic acid method. The ELISA was compared to the haemagglutination-inhibition (HI) test for antibodies to NDV. ELISA cut-off point was established through TG-ROC analysis. Total correlation was observed between the ELISA and the HI, being the ELISA efficient in the identification of positive and negative sera, with high sensitivity and specificity (100%). These results validate the use of the indirect ELISA as an alternative for the detection of NDV-specific IgG in japanese quail sera, with the advantage of high sensitivity and automation.
Tipo: | Info:eu-repo/semantics/other
Idioma: | Português
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352007000500040
Editor: | Universidade Federal de Minas Gerais, Escola de Veterinária
Relação: | 10.1590/S0102-09352007000500040
Formato: | text/html
Fonte: | Arquivo Brasileiro de Medicina Veterinária e Zootecnia  v.59 n.5 2007
Fonte: | info:eu-repo/semantics/openAccess