Data/hora: 01/06/2024 01:15:23

Provedor de dados: | 45
País: | Brazil
Título: | Nova técnica para contagem do número de células de blastocistos
Autores: | Costa,E.P.; Lopes,F.G.; Pereira,E.C.M.; Queiroz,V.L.D.; Macedo,G.G.; Almeida Neto,J.R.M.; Costa,A.H.A.
Data: | 2010-12-01
Ano: | 2010
Palavras-chave: | Contagem de células; Embrião; Reprodução.
Resumo: | A simplified, fast, and innovative method was developed to count the total cell number in blastocysts. Murine blastocysts (N = 195) were used in this study. They were obtained after 10h culture of initial blastocysts, compact morulae grades I and II recovered from superovulated mouse. After culture, the blastococysts were selected to test the new proposal of counting. The process was done after embryo fixation in a sodium citrate solution, and adherence in glass slide. Following, the coloration was done using a fast panoptic coloration kit. As a result, it was possible to identify the blastomeres and count them in each blastocyst. This method provided a fast and effective analysis of the total cell number when compared with other techniques. Moreover, this new method shows advantages related to the cell visualization, which can be done in more simple equipment like stereoscopic microscope. Other interesting observed point was the long period of time and quality that the coloration stays on slides, considering other techniques.
Tipo: | Info:eu-repo/semantics/other
Idioma: | Português
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352010000600032
Editor: | Universidade Federal de Minas Gerais, Escola de Veterinária
Relação: | 10.1590/S0102-09352010000600032
Formato: | text/html
Fonte: | Arquivo Brasileiro de Medicina Veterinária e Zootecnia  v.62 n.6 2010
Fonte: | info:eu-repo/semantics/openAccess