Data/hora: 09/06/2024 15:54:47

Provedor de dados: | 45
País: | Brazil
Título: | Bovine viral diarrhea diagnostic: immunohistochemistry standardization for routine
Autores: | Andrade,G.I.; Serra,C.V.; Barbosa-Stancioli,E.F.; Serakides,R.; Lobato,Z.I.P.
Data: | 2005-10-01
Ano: | 2005
Palavras-chave: | Bovino; Diarréia bovina a vírus; Imunoistoquímica; Bovine viral diarrhea; Virus; Immunohistochemistry.
Resumo: | The immunohistochemistry standardization for bovine viral diarrhea virus (BVDV) diagnostic was described. The formalin-fixed tissue samples from a heifer with mucosal disease were used as positive control. The validation of the first phase results was performed using samples from an aborted fetus and a calf infected with reference strains of BVDV. The best results were seen using monoclonal antibodies and a commercial kit consisting of labelled streptavidin biotin (LSAB) reagents and the diaminobenzidine (DAB) substrate-chromogen reagent. The immunohistochemistry demonstrated to be an useful method for routine diagnosis for the controll and detection of BVDV infection.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352005000500018
Editor: | Universidade Federal de Minas Gerais, Escola de Veterinária
Relação: | 10.1590/S0102-09352005000500018
Formato: | text/html
Fonte: | Arquivo Brasileiro de Medicina Veterinária e Zootecnia  v.57 n.5 2005
Fonte: | info:eu-repo/semantics/openAccess