Data/hora: 05/06/2024 03:48:03

Provedor de dados: | 45
País: | Brazil
Título: | Validation of an HPLC-UV method for the identification and quantification of bioactive amines in chicken meat
Autores: | Assis,D.C.S.; Menezes,L.D.M.; Lima,A.L.; Klein,R.W.T.; Heneine,L.G.D.; Lara,L.J.C.; Teixeira,L.V.; Ornellas,C.B.D.; Cançado,S.V.; Figueiredo,T.C.
Data: | 2016-06-01
Ano: | 2016
Palavras-chave: | Validation; Chicken meat; Bioactive amines; HPLC-UV.
Resumo: | ABSTRACT A high-performance liquid chromatography with ultraviolet detection (HPLC-UV) method was validated for the study of bioactive amines in chicken meat. A gradient elution system with an ultraviolet detector was used after extraction with trichloroacetic acid and pre-column derivatization with dansyl chloride. Putrescine, cadaverine, histamine, tyramine, spermidine, and spermine standards were used for the evaluation of the following performance parameters: selectivity, linearity, precision, recovery, limits of detection, limits of quantification and ruggedness. The results indicated excellent selectivity, separation of all amines, a coefficient of determination greater than 0.99 and recovery from 92.25 to 102.25% at the concentration of 47.2mg.kg-1, with a limit of detection at 0.3mg.kg-1 and a limit of quantification at 0.9mg.kg-1 for all amines, with the exception of histamine, which exhibited the limit of quantification, of 1mg.kg-1. In conclusion, the performance parameters demonstrated adequacy of the method for the detection and quantification of bioactive amines in chicken meat.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352016000300805
Editor: | Universidade Federal de Minas Gerais, Escola de Veterinária
Relação: | 10.1590/1678-4162-8668
Formato: | text/html
Fonte: | Arquivo Brasileiro de Medicina Veterinária e Zootecnia  v.68 n.3 2016
Fonte: | info:eu-repo/semantics/openAccess