Data/hora: 07/06/2024 08:25:27

Provedor de dados: 	45	
País: 	Brazil	
Título: 	Detection of equine herpesvirus 1 DNA in a single embryo and in horse semen by polymerase chain reaction	
Autores: 	Carvalho,R.; Passos,L.M.F.; Oliveira,A.M.; Henry,M.; Martins,A.S.	
Data: 	2000-08-01	
Ano: 	2000	
Palavras-chave: 	Equine; Semen; Embryo; EHV-1; EHV-4; PCR.	
Resumo: 	The genome of one equine embryo and three equine semen specimens collected from a Brazilian farm were tested by polymerase chain reaction (PCR) for the presence of EHV-1 and EHV-4-specific timidine kinase (TK) sequences. The PCR detected specific EHV-1 TK gene sequences in all samples tested. The peripheral blood leukocytes (PBL) of the embryo donor mare also was amplified by EHV-1 TK primers. Infectious virus was not recovered from any specimens. The animals did not show any clinical signal of EHV-1 or EHV-4 infections. EHV-4 was not detected in the studied specimens. The results indicate that PCR was more sensitive than virus isolation in cell culture for detecting EHV-1 in semen of carrier horses.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352000000400002	
Editor: 	Universidade Federal de Minas Gerais, Escola de Veterinária	
Relação: 	10.1590/S0102-09352000000400002	
Formato: 	text/html	
Fonte: 	Arquivo Brasileiro de Medicina Veterinária e Zootecnia  v.52 n.4 2000	
Fonte: 	info:eu-repo/semantics/openAccess